Analysis of the genetic stucture of certain types of scaled carp / Аналіз генетичної структури окремих типів українського лускатого коропа
Journal Title: Рибогосподарська наука України - Year 2016, Vol 38, Issue 4
Abstract
Purpose. Study of the genetic structure specificity, intra- and interpopulation genetic variation of the brood stocks of Ukrainian scaled carp from different fish farms of Ukraine using DNA markers (ISSR-PCR). Methodology. A PCR (ISSR-PCR) method with appropriately selected primers has been used to investigate the genetic structure specificity. Findings. The genetic structure analysis of Ukrainian scaled carp has been carried out using three microsatellite DNA loci: (AGC)6G, (AСC)6G, (AGC)6С. In total, 55 amplicons were detected for all primers. Fifteen primers were detected when using (AGC)6G primer, 17 for (AСC)6G primer and 23 for (AGC)6С primer. The molecular weight on electropherograms had maximum values when using (AСC)6G primer (500 n.p. – 3500 n.p.) in Nyvky carp. The detected specific differences between the examined populations of scaled carp depend on their genetic origin. Variations of the detected amplicons are sufficient for separating individual fish or, if works are carried out with a group of broodfish, for selecting parental pairs for increasing genetic diversity. Originality. The use of ISSR-markers allowed detecting the peculiarities of the genetic structure, level of genetic variability of the brood stocks of Ukrainian scaled carp. For the first time, we obtained new data on the specificity of the genetic structure when using PCR techniques, which allow detecting specific mechanisms of the maintenance of the relative stability of the genetic material of Ukrainian scaled carps and allow controlling and preserving the specificity of their genetic structure. Practical value. Proposition of a method for the genetic control of Ukrainian scaled carp stocks based on the application of PCR techniques, which allows performing an analysis of the genetic structure of fish stocks and using the genetic information at early stages of breeding selection process.
Authors and Affiliations
А. Маriutsa, S. Tarasjuk, І. Hrytsyniak
Keywords
Related Articles
- EP ID EP474965
- DOI 10.15407/fsu2016.04.113
- Views 48
- Downloads 0
MORPHOPHYSIOLOGICAL PARAMETERS OF SOME STURGEON SPECIES (ACIPENSERIDAE, ACIPENSERIFORMES) OF DIFFERENT AGE GROUPS REARED IN AQUACULTURE / МОРФОФІЗІОЛОГІЧНІ ПОКАЗНИКИ ДЕЯКИХ ВИДІВ ОСЕТРОВИХ РИБ (ACIPENSERIDAE, ACIPENSERIFORMES) РІЗНОГО ВІКУ, ЩО ВИРОЩУЮТЬСЯ В АКВАКУЛЬТУРІ
Purpose. To study some morphometric parameters, protein and lysozyme content in organs and serum in several sturgeon species reared in aquiculture at the ages of 3, 8 and 18 months, and to investigate the specific and ag...
EFFECT OF NON-ESSENTIAL ELEMENTS (MERCURY. ARSENIC) ON SALMONIDS (SALMONIDAE) (REVIEW) / ВПЛИВ НЕЕСЕНЦІАЛЬНИХ ЕЛЕМЕНТІВ (РТУТЬ, МИШ’ЯК) НА ОРГАНІЗМ ЛОСОСЕВИХ (SALMONIDAE) РИБ (ОГЛЯД)
Purpose. The problem of water ecosystem pollution with heavy metals achieved great actuality during recent years, both because of their significant distribution in environment, and wide spectrum of their toxic effects on...
MORPHOMETRIC CHARACTERISTICS OF THE REPLACEMENT-BROOD STOCK OF RAINBOW TROUT (ONCORHYNCHUS MYKISS (WALBAUM, 1792) REARED IN THE CONDITIONS OF THE INDUSTRIAL FISH FARM "SLOBODA-BANYLIV” / МОРФОМЕТРИЧНА ХАРАКТЕРИСТИКА РЕМОНТНО-МАТОЧНОГО СТАДА РАЙДУЖНОЇ ФОРЕЛІ (ONCORHYNCHUS MYKISS (WALBAUM, 1792) ВИРОЩЕНОЇ В УМОВАХ ІНДУСТРІАЛЬНОГО ГОСПОДАРСТВА «СЛОБОДА БАНИЛІВ»
Purpose. To analyze the results of morphometric measurements and dates of aquaculture-biological characteristics of the young-of-the-year and age-1+ rainbow trout reared under industrial technology at instable conditions...
Biological characteristics of silver bream (Blicca bjoerkna) in commercial fishery in the Kyiv reservoir / Біологічна характеристика проскирки (Blicca bjoerkna), як об’єкту промислового рибальства у Київському водосховищі
Purpose. The determination and analysis of main biological characteristics of silver bream from the point of view of the formation and exploitation of its commercial stock in the Kyiv reservoir. Methodology. The work is...
The cultivation of the nematodes Panagrellus, Turbatrix (Anguillula) and Rhabditis for using in fish feeding / Культивування нематод з родів Panagrellus, Turbatrix (Anguillula) і Rhabditis для використання в годівлі риб
Purpose. To analyze professional literature and summarize the information on the cultivation of the representatives of three nematode (Nematoda) genera. To highlight the optimal biotechnology of the cultivation of banana...