Androgen receptor inhibitor stimulates telomerase activity of pig granulosa cells in vitro
Journal Title: Animal Science Papers and Reports - Year 2011, Vol 29, Issue 4
Abstract
The aim of the study was to investigate the effect of testosterone (T) and dihydrotestosterone (DHT) applied alone or in combination with antiandrogen (flutamide) on proliferation, progesterone secretion and telomerase activity (TA) of pig granulosa cells (GC) derived from small (1-3 mm; SFGC) and large (5-7 mm; LF-GC) ovarian follicles. Cells were treated with investigated factors for 48 h. 5α-DHT stimulated (P<0.05-0.01) proliferation of SF and LF granulosa cells. Flutamide applied individually and in a combination with testosterone and DHT stimulated (P<0.05-0.01) proliferation of pig GC from small and large antral follicles. Flutamide had no effect on progesterone synthesis in small as well as in large follicle GC. Antiandrogen applied individually and in a combination with testosterone and DHT enhanced (P<0.05-0.01) telomerase actitvity in SF- and LF-GC. The results of the study suggest the involvement of androgen receptor in telomerase activity regulation in pig GC and a link between telomerase and the proliferation status of GC.
Authors and Affiliations
Ewa Chronowska, Tomas Kott
Keywords
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