Antibacterial Activity of Actinomycetes Isolated from Waste Dump Soil from Western Uganda
Journal Title: Microbiology Research Journal International - Year 2017, Vol 21, Issue 5
Abstract
Aims: To determine the antibacterial activity of actinomycetes isolated from waste dump soil in Western Uganda. Study Design: The study was an experimental laboratory design. Place and Duration of Study: Waste dump soil samples were collected from Bushenyi, Kabale, Kasese, and Mbarara districts in Western Uganda, from May, 2016 to February, 2017. Methodology: Actinomycetes were isolated from 22 waste dump soil samples collected using standard spread plate technique, all isolates were screened primarily using cross streak method against Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853, and Staphylococcus aureus ATCC25923, resistant clinical bacteria: Escherichia coli 2966, Pseudomonas aeruginosa 2929, and Staphylococcus aureus 2876 (MRSA). Secondary screening was carried out by first growing all fifty six isolates (56) in broth media and their supernatants were tested for activity using agar well diffusion method. The remaining broths were extracted using ethanol. The ethanol extract was also tested for antibacterial activity using agar well diffusion method. The MIC was determined using tubes dilution technique and MBC was determined by culture method. Results: Fifty six (56) actinomycetes isolates were isolated from 22 waste dump soil samples. Four (7.14%) isolates showed activity to at least one test bacteria during primary screening. Eight (14.29%) actinomycetes isolates fermented broth showed activity to at least one test bacteria during secondary screening, with mean zone of inhibition of 7.67±1.45 to 33.67±2.03 mm. Isolate KBMWDSb6 showed activity to all test bacteria with exception of resistant Staphylococcus aureus 2876 (MRSA) while isolates BRWDSc (SP) and KBRWDSa3 (RF) showed activity to all sensitive standard isolates and resistant Staphylococcus aureus 2876 (MRSA). Eleven (19.64%) actinomycets isolates ethanol extracts showed activity to at least one test bacteria with mean zone of inhibition 7.33±1.20 to 31.67±1.45 mm. The MIC and MBC of the extracts were found to be 0.07 to 0.62mg/mL and 0.15 to 1.25mg/mL respectively. Conclusion: The findings of this study indicated that actinomycetes spp isolated from waste dump soil collected from Western Uganda have ability to produce bioactive compounds with activity against bacteria including clinically drugs resistant bacterial isolates. This could be a good source of novel antibiotics.
Authors and Affiliations
Adamu Almustapha Aliero, Eilu Emmanue, Maniga Nyabayo Josephat, Sambo Haruna Aliyu, Matilda Angela Okech, John Odda
Keywords
Related Articles
- EP ID EP316016
- DOI 10.9734/MRJI/2017/36459
- Views 96
- Downloads 0
Identification of Bacteria Isolated from Crude Oil Polluted Fish Pond Undergoing Bioremediation
The study was carried out to identify bacteria associated with crude oil polluted water collected from the Fish pond, Department of Fisheries and Aquaculture, Federal University of Technology Akure, Ondo State Nigeria. T...
Level of Awareness of Hepatitis B Viral Infection among a Subset of Makurdi Community in Benue State, Nigeria
Background: The high prevalence of chronic hepatitis B virus (HBV) carriers worldwide coupled with complications such as liver cirrhosis and hepatocellular carcinoma makes the disease a global health challenge. Aim: To...
Characterization of Vaginal Lactobacillus Strains and Their Potential Antagonistic Effects on Candida albicans
Aims: To characterize Lactobacillus strains for potential beneficial effects, especially for inhibition of Candida albicans growth. Materials and Methods: Twelve vaginal Lactobacillus strains belonging to five species fr...
Antimicrobial Activity of Moringa oleifera Leaf against Isolates of Beef Offal
This study was designed to evaluate the antimicrobial potentials of ethanol and aqueous extracts of Moringa oleifera against microorganisms isolated from beef offal. The beef offal samples (liver, kidney and intestine) w...
Amylolytic Potential of Lactic Acid Bacteria Isolated from Wet Milled Cereals, Cassava Flour and Fruits
Aims: This research was conducted with the objective to evaluate amylolytic potential of lactic acid bacteria isolated from wet milled cereals, cassava flour and fruits. Methodology: Strains of lactic acid bacteria were...