ANTICANCER POTENTIAL OF METHANOLIC AND AQUEOUS EXTRACT OF LEUCAS INDICA (LINN.) AGAINST EHRLICH ASCITES CARCINOMA CELLS ON SWISS ALBINO MICE
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 6
Abstract
The present study was designed to evaluate the in vivo anticancer activity of methanolic and aqueous extract of Leucas indica Linn. (Aerial parts) against Ehrlich’s Ascites Carcinoma (EAC) on Swiss albino mice. The anticancer activity was done by intra peritoneal transplantation of EAC cells into mice and subsequently analysis of ascitic fluid, haematological, biochemical and histopathological parameters along with body weight and percentage of life span. The animals were treated with the extracts at the dose level of 200 and 400 mg/kg, intra peritoneally and compared to the reference drug vinblastine. Both the extracts showed significant dose dependent reduction of viable cells (cancer cells), increased non-viable cells (dead cells), decreased in body weight and increased the duration of life span. In case of hematological study the extracts showed increased hemoglobin level and RBC count but decreased WBC count. The certain important serum enzymes like glutamate oxaloacetate transaminase, glutamate pyruvate transaminase and alkaline phosphatase levels were also decreased in treated group of animals. Finally, in case of histopathological investigation of livers showed a significant protection and regeneration of EAC induced oxidative stress and cellular damage. The preliminary phytochemical screening of the extracts revealed the presence of flavonoids, total phenolic compounds, saponin and tannin. However the aqueous fraction of the Leucas indica possesses more potential anticancer property compared to methanolic fraction. The anticancer property of this plant was considered due to the presence of flavonoids and total phenolic compounds.
Authors and Affiliations
Mahananda Sarkar , Mousumi Das , Debmalya Mitra , Aditya Jena , Arnab De , Amalesh Samanta
Keywords
Related Articles
- EP ID EP151503
- DOI 10.7897/2230-8407.04641
- Views 103
- Downloads 0
SYNTHESIS AND BIOLOGICAL ACTIVITY OF BUTANONE THIOSEMICARBAZONE AND THEIR METALLIC COMPLEXES
In present work, Ligand butanone thiosemicarbazone and their 11 complexes of the type ML2X2, ML2X’, where M = Cu(II), Cd(II), Co(II), Zn(II), Hg(II); L = butanone thiosemicarbazone; X = Cl, NO3 or CH3COO; X’ = SO4 hav...
EVALUATION OF WOUND HEALING ACTIVITY OF HELIOTROPIUM INDICUM LEAVES
Present study is about the wound healing activity of ethanol and aqueous extracts of H.indicum leaves in wistar rats. Three wound models viz incision, excision and dead space wound were used in this study. The biophysica...
PREPARATION AND EVALUATION OF HERBAL DENTIFRICE
Dentifrices are the products which are used to maintain the oral hygiene such as freshness of mouth and to avoid tooth decay. The oral hygiene can be maintained throughout the day by using various dentifrices prepared by...
SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF MONTELUKAST SODIUM AND BAMBUTEROL HYDROCHLORIDE IN TABLETS
The present manuscript describe simple, sensitive, rapid, accurate, precise and economical first derivative spectrophotometric method for the simultaneous determination of montelukast sodium and bambuterol hydrochloride...
SYNTHESIS OF 6, 7-DISUBSTITUTED-2-ARYL-4H-1-BENZOPYRAN-4-ONES AS ANTIBACTERIAL AGENTS
Benzopyrones constitute one of the major classes of natural products. Both semisynthetic and natural benzopyrone derivatives are known to possess important biological properties and as a result several attempts and proce...