Antimicrobial Effects of Platelet-rich Plasma against Selected Oral and Periodontal Pathogens
Journal Title: Polish Journal of Microbiology - Year 2017, Vol 66, Issue 1
Abstract
Antimicrobial properties of platelet rich plasma (PRP) against various microorganisms have been recently pointed out. PRP could be an alternative to conventional antibiotics in preventing oral and periodontal infections. We examined whether PRP has in vitro antimicrobial properties against Aggregatibacter actinomycetemcomitans, Prophyromonas gingivalis, Staphylococcus aureus and Candida albicans. PRP and platelet-poor plasma (PPP) were obtained from whole blood of 10 healthy volunteers and 10 periodontitis patients. In vitro laboratory susceptibility was carried out using the modified agar diffusion method by measuring the diameters of inhibition zones on agar plates coated with selected microbial strains. Both calcium chloride (CaCl2) activated and non activated samples were tested. Both activated PRP and PPP, of both patients and controls, effectively inhibited the growth of A. actinomycetemcomitans, P. gingivalis and C. albicans. However, a statistically significant difference in favor of PRP was found indicating more susceptibility to PRP than PPP (p < 0.05). Non activated PRP and PPP exhibited negative zones of inhibition against the studied microorganisms. There was no activity against S. aureus. No statistically significant difference was found between the antimicrobial effects of PRP and/or PPP obtained from patients and controls (p > 0.05). We conclude that PRP is a potentially useful substance against oral and periodontal pathogens. Activated PRP was found to be more active than activated PPP and the activation of coagulation is a fundamental step. Additionally, the antimicrobial activity of PRP and/or PPP seems not to be affected by periodontitis.
Authors and Affiliations
Reham L. Aggour, Lina Gamil
Keywords
Related Articles
- EP ID EP225719
- DOI -
- Views 94
- Downloads 0
<strong>Identification and Localization of β-D-Glucosidase from Two Typical <em>Oenococcus oeni </em>Strains</strong>
β-D-glucosidase (βG) gene from [i]Oenococcus oeni [/i]SD-2a and 31MBR was cloned, sequenced and analyzed, also intracellular βG of the two strains was further localized. Results showed that βG gene of the two strains was...
The Prevalence of Campylobacter spp. in Polish Poultry Meat
The prevalence, count and molecular identification of Campylobacter spp. in Polish poultry meat were analysed. 181 samples of meat from chicken (70), turkey (47), duck (54) and goose (10) were studied. Campylobacter spp....
Gene Expression during BTEX Biodegradation by a Microbial Consortium Acclimatized to Unleaded Gasoline and a Pseudomonas putida Strain (HM346961) Isolated from It
Pseudomonas putida strain (HM346961) was isolated from a consortium of bacteria acclimatized to unleaded gasoline-contaminated water. The consortium can efficiently remove benzene, toluene, ethylbenzene and xylene (BTEX)...
<strong>Biochemical and Molecular Characterization of Carotenogenic Flavobacterial Isolates from Marine Waters</strong>
Carotenoids are known to possess immense nutraceutical properties and microorganisms are continuously being explored as natural source for production of carotenoids. In this study, pigmented bacteria belonging to [i]Flav...
In Vitro Studies of Antibacterial and Antifungal Wound Dressings Comprising H<sub>2</sub>TiO<sub>3</sub> and SiO<sub>2</sub> Nanoparticles
The incidence rate of the infected and complex wound is established approximately 40 000/1 million of the world’s adult population. The aim of this study was to assess the efficiency of three novel types of wound dr...