Antimicrobial photodynamic therapy on Candida albicans on acrylic surfaces of dental prostheses. In vitro study
Journal Title: Revista Eugenio Espejo - Year 2022, Vol 16, Issue 3
Abstract
Species of the genus Candida are frequently found in the oral microbiota of humans. Objective: to compare the antimicrobial effectiveness of photodynamic therapy on Candida albicansstrains on acrylic surfaces for dental prostheses by using a 660nm wavelength laser and methylene blue as a photosensitizing agent, with respect to other therapeutic methods. Methodology: in vitro study, using 60 thermosetting acrylic discs immersed in a suspension of C. albicans, generating a biofilm simulation on the surface of a dental prosthesis. After that, five random groups of 12 units were formed to apply them different therapeutic procedures: G1 (saline solution), G2 (chlorhexidine 0.12%), G3 (nystatin in topical solution at 0.001ml/ul), G4 (0.005% methylene blue + diode laser with λ=660nm, 100mW, 32J/cm2) and G5 (0.01% methylene blue + diode laser with λ=660nm, 100mW, 321J/cm2). Results: a statistically significant difference was observed when comparing groups G4 and G5 with respect to G1 and G3 (p<0.05). Conclusion: the efficacy in reducing the number of viable C. albicans CFU was higher in the groups where laser radiation with a wavelength of 660nm was used with different concentrations of methylene blue. The use of nystatin and physiological saline had the lowest efficacy values.
Authors and Affiliations
María Alejandra Jarrín Ríos, Maria Fernanda Caicedo Breeddy, Pablo Ruben Garrido Villavicencio, Héctor Eduardo Cepeda Inca
Keywords
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- EP ID EP708782
- DOI https://doi.org/10.37135/ee.04.15.08
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