ATTENUATING EFFECT OF TRITERPENOID SAPONIN RICH FRACTION OF ACHYRANTHES ASPERA LINN. ON ACUTE AND CHRONIC INFLAMMATION IN EXPERIMENTAL RATS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 7
Abstract
Objective: Achyranthes aspera Linn. is used as a traditional remedy for the treatment of various inflammatory conditions in India. The present study was designed to investigate the anti-inflammatory activity of methanolic extract of Achyranthes aspera (AA) and its active fraction using bioassay guided fractionation.Methods: The dry whole plant of AA was extracted with methanol and then fractionated with different polarity of solvents. Bioactive petroleum ether fraction was re-fractionated into triterpenoid saponin rich (TSR) and non-saponin subfractions and tested for anti-inflammatory activity. The activity of TSR subfraction was evaluated against oxidative stress induced by carrageenan in rat paw tissues.Results: TSR fraction showed significant (p<0.05) inhibition of rat paw oedema volume. Moreover, TSR fraction was also found to attenuate carrageenan induced oxidative damage by improving antioxidant enzymes levels. Furthermore, TSR fraction inhibited both heat and hypotonicity induced haemolysis of erythrocytes in vitro. In addition, TSR fraction significantly (p<0.05) reduced the granuloma formation in cotton pellet-induced granuloma in rats.Conclusions: The present study indicates TSR is a potential therapeutic for the treatment of inflammation-associated disorders.Â
Authors and Affiliations
Pankaj S. Kothavade, Vipin D. Bulani, Archana R. Juvekar
Keywords
Related Articles
- EP ID EP578536
- DOI -
- Views 59
- Downloads 0
PROTEOLYTIC AND MILK CLOTTING ACTIVITY OF FRACTIONATED PROTEIN OF CITRULLUS LANATUS
Objective: The objective of the present investigation was to evaluate milk clotting activity as well as proteolytic activity of protein fractions of seeds of Citrullus lanatus.Methods: Storage proteins were extracted by...
ZERO, FIRST, SECOND ORDER DERIVATIVE AND AREA UNDER CURVE SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF CEFIXIME TRIHYDRATE IN PHARMACEUTICAL FORMULATION
Objective: A simple, accurate, precise and specific zero, first, second order and area under curve spectrophotometric methods has been developed for determination of Cefixime Trihydrate in its tablet dosage form by using...
OLEOGELS OF OLIVE OIL AND SOYBEAN OIL FOR TOPICAL DRUG DELIVERY: A COMPARATIVE ANALYSIS
Objective: The objective of the present investigation was to develop olive and soybean oil-based oleogels with Span 40 and/or Tween 80 (as gelator and/or surfactant) and determine the critical gelator concentration (CGC)...
QUALITY CONTROL EVALUATION OF BRANDS OF MEBENDAZOLE 100 mg TABLETS ON THE ILLEGITIMATE PHARMACY OUTLETS
Objective: The objective of the present study is to assess physical properties and the quality control parameters of the marketed brands of Mebendazole 100 mg found in illegitimate pharmacy outletsMethods: Five brands of...
GREEN SYNTHESIS OF SILVER NANOPARTICLE FROM LEAF EXTRACT OF AEGLE MARMELOS AND EVALUATION OF ITS ANTIBACTERIAL ACTIVITY
Objective: The synthesis of metal nanoparticle is a growing area of research in modern material science and technology. Utilization of the silver nanoparticles in the field of biomedical nanotechnology and nanomedicines...