Authentication of traditional crop Kalongi (Nigella sativa L.) by LAMP marker
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 6
Abstract
Nigella sativa, commonly known as kalongi is an important drug of traditional system of medicine, commonly used against cough, fever, abdominal disorders, skin infections, paralysis and jaundice. Because of increased demand and high price, there are chances that the herb could be adulterated in the trade by other related species. Therefore, a reliable authentication method is needed to facilitate identification of this genuine material from its adulterants. To carry out the work, the market samples were procured from the crude-drug markets of different geographical regions of India. The RAPD derived LAMP technique was employed for the characterization of different accessions of Kalongi. 19 primers were used of which one unique band, common in all accessions were eluted, cloned and sequenced. LAMP primers were designed and LAMP product formation was detected at 60°C.Out of 25 primers, 19 primers amplified a total of 524 reproducible, clear and scorable bands. One monomorphic RAPD fragment present in all the accessions, amplified by OPAA-09 primer, was developed into LAMP marker for identification of N. Sativa. The primers successfully amplified the genome of kalongi while as in negative control (Catharanthus roseus) there was no amplification. The LAMP markers developed in this study may provide guidance for the authentication of plant materials traded as Kalongi.
Authors and Affiliations
Showkat Hussain Ganie, Deepak Yadav, Altaf Ahmad, Anis Chadhry, Mohd Asif
Keywords
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