Calcium antagonist of n-butanol fraction (BuF) from the stem bark of Terminalia superba Engl. et Diels (Combretaceae) on rabbit duodenum
Journal Title: Scholars Academic Journal of Pharmacy - Year 2014, Vol 3, Issue 1
Abstract
The Objective of the present study was to investigate the pharmacological effects and the possible mechanism (s) that mediated the effects of the organic fraction from n-butanol-water partiton (50:50 v/v) of the stem bark of Terminalia superba and to screen for the presence of phytochemical constituents which can be responsible for these observations. Organic n-butanol fraction (BuF) from the stem bark of Terminalia superba was evaluated for its capacity to induce spasmolytic effect on spontaneous contraction of isolated rabbit duodenum as well as for its possible calcium blocking actions. Pieces of rabbit duodenum (3cm) were mounted in an organ bath containing air bubbled Mac Ewen solution with 1g initial tension. The non cumulative concentrations of BuF exhibited a significant (p<0.05) relaxation on the spontaneous contraction of isolated rabbit duodenum for concentrations ranging from 0.02 to 0.08 mg/ml. The pre-contracted duodenum by ACh (2.20x10-6mg/ml), KCl (2.43 x10-6mg/ml) and BaCl2 (2.30 x10-6mg/ml) were relaxed by the non cumulative concentrations (0.02, 0.04 and 0.08 mg/ml) of BuF (p<0.05). In high potassium (2.43 x10-6mg/ml) Ca2+-free Mac Ewen solution, non cumulative concentration of CaCl2 (2.02 x10-3µg/ml) induced duodenum contractions. However, the non cumulative concentrations of BuF (0.02, 0.04 and 0.08 mg/ml) reduced these CaCl2-induced contractions concentration-dependently (p<0.05). These results suggest that BuF antispasmodic effect on rabbit duodenum may be due to blockade of voltage dependent calcium channels. Tannins, flavonoids, quinones, saponins, reduced sugar, sterols and polyterpenes were found out as major active constituents of the tested extract. Some of these phytochemical constituents such as tannins, flavonoids, saponins, sterols and polyterpenes which are known for their antispasmodic effect may explain the relaxant actions of BuF. Keywords: Antispasmodic, Terminalia superba, rabbit, duodenum.
Authors and Affiliations
Nomane Bernard Goze, Kouakou Léandre Kouakou, Nahounou Mathieu Bléyéré, Brou André Konan, Kouao Augustin Amonkan, Kouakou Jean Claude Abo, Ehouan Etienne Ehilé
Keywords
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