CHARACTERIZATION AND EVALUATION OF ANTIOXIDANT ACTIVITY OF OCIMUM CANUM LEAVES AND ITS EFFECIENCY ON SCHISTOSOMA MANSONI LARVAL STAGE
Journal Title: Indo American Journal of Pharmaceutical Research - Year 2017, Vol 7, Issue
Abstract
Schistosomiasis is the most endemic disease caused by the genus Schistosoma. Its control is dependent on killing the worm itself or one of its larval stages. The objective of this study is to determine the total phenolic and flavonoid contents, evaluating the antioxidant activity of Ocimum canum leaf methanol extract by DPPH and ABTS as well as investigating the efficiency of plant extract against Schistosoma mansoni larval stages. Also, GC-MS analysis of plant extract was carried out. The results revealed that Ocimum canum leaf methanol extract showed high total phenolic content (321.78±0.69 mg GAE/g extract) and flavonoid content (71.64±10.66 mg RE/g extract). Furthermore, there is a significant correlation between the concentration of the extract and the inhibition percentage of the free radicals. The higher inhibition percentage of plant extract was recorded by DPPH than ABTS at 500?g/ml (84% and 62%) respectively. Also, IC50 of DPPH and ABTS (26.41±1.39 and 194.45±2.03) respectively. Regarding to ceracricidal and miracidicidal activity, the plant extract showed larvicidal activity as it killed 82% of cercariae while, it was completely lethal to miracidia at 500ppm within 2 hours. On the other hand, 36 chemical compounds were identified from plant extract. The main components are Phytol (23%), Hexadecanoic acid, methyl ester (15.48%), 9,12,15-Octadecatrienoic acid methyl ester (14.99%), n-Hexadecanoic acid (7.62%), 9,12,15-Octadecatrienoic acid (5.04%), Ethyl linoleate (3.58%) and 2-Propenoic acid, 3-phenyl-, methyl ester (3.17%). This study suggested that Ocimum canum can be used as promising plant to control schistosomiasis disease as well as its potential antioxidant activity.
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF CIPROFLOXACIN HYDROCHLORIDE AND FLUOCINOLONE ACETONIDE IN THEIR SYNTHETIC MIXTURE
Abstract The present research work aims to develop a simple, precise, accurate, rapid, reproducible and economical method for the estimation of Fluocinolone acetonide and Ciprofloxacin hydrochloride in their synthetic mi...
FLOATING MULTIPARTICULATE SYSTEMS: A NOVEL APPROACH IN GASTRORETENTIVE DRUG DELIVERY SYSTEMS
Abstract Recent advances in novel drug delivery system to enhance the safety and efficacy of the drug molecule by formulating a dosage form being convenient for administration. A controlled drug delivery system with prol...
Matrix Tablets as Controlled drug delivery systems
From the point of patient compliance, dosage regimen of an orally administered drug may be considered to be optional when the therapeutic effects are maintained for the desired duration of the treatment at the lowest fre...
FORMULATION AND EVALUATION OF SUSTAINED RELEASE MATRIX TABLETS OF CAPTOPRIL
The present investigation concerns the development of Sustained release matrix tablets of Captopril, which after oral administration are designed to prolong the duration up to 12 hrs and thereby increase patient complian...
DEVELOPMENT AND EVALUATION OF EMULGEL FOR WOUND HEALING ACTIVITY
Abstract The study was designed with the aim to evaluate metformin and atorvastatin for its wound Healing activity.Two different formulations of Metformin emulgel and atorvastatin emulgel were formulated using Design exp...