Characterization of Biosurfactant Produced by Pseudomonas putida PS6 Isolated from Contaminated Soil
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 5
Abstract
Biosurfactants are surface active compounds that reduce the interfacial tension between two liquids or between a liquid and solid, these compounds produce extracellularly or as part of the cell membrane in bacteria, yeast and fungi. In this study the Pseudomonas putida PS6 isolated from soil contaminated with hydrocarbons and it showed the highest capability to produce the biosurfactant when grown in BH media with 1% of diesel oil as carbon source. The biosurfactant was extracted from cell-free supernatant of Pseudomonas putida PS6 culture by chloroform: methanol (2:1) and then identified based on the methods of TLC, chemical compositions, blue agar plate and FTIR analysis. The results from all methods indicate that the biosurfactant can designated as glycolipid. The biosurfactant showed emulsification activity on a wide range of temperature between (20-100˚C) and gave the highest emulsification index 74.6% at pH 7, while the stability of biosurfactant decreased at acidic and alkaline conditions, also in the study the effect of salinity, the produced biosurfactant was stable in different concentration of NaCl between 5-15%. This biosurfactant showed high stability under studied conditions, therefore can be used in industrial applications.
Authors and Affiliations
Zainab Hani Hatef, Saad Hussain Khudeir
Keywords
Related Articles
- EP ID EP392108
- DOI 10.9790/3008-1206011318
- Views 77
- Downloads 0
Characterization and Antimicrobial Activity of AgNPs Synthesized by Streptomyces sp. RHS16 Against Fish Pathogens
Silver nanoparticles (AgNPs) attracted great attention due to their unusual and fascinating properties, and applications in many fields. This work reports the fabrication of AgNPs by a newly isolated Streptomyces sp. RHS...
Influence of L-arginine on Cisplatin-Induced Hepatotoxicity in both normally Fed and Protein Malnourished Rats Medhat I. Abd-El-Hamid1 , Azza A. Ali2 , Hebatalla I. Ahmed2 , Mohammad
Liver has great capacity to detoxify toxic substances and synthesized useful principles. Therefore, damage to the liver inflicted by hepatotoxic agents is of grave consequences. Protein malnutrition produces profound eff...
Antimicrobial Activity of Single And Combined Extracts of Medicinal Plants From Cameroon
Selected Cameroonian plants have been investigated for their antifungal and antibacterial activity against five species: four bacteria, namely S. aureus, S. pyogenes, S. mutans, Pseudomonas aeruginosa and the yeast C. al...
Prevalence of vitamin D deficiency in adults in the coastal regions of Odisha, India
Background and objectives: Vitamin D is a fat-soluble vitamin belonging to secosteriods. It is responsible for intestinal absorption and bioavailibity of calcium and phosphorus.Synthesisof vitamin D in response to ultrav...
Stephania glabra (Menispermaceae)- Phytochemical Perspective.
Background- Stephania glabra (Roxb) has long been used for the treatment of asthma with several other valuable uses. Over 150 alkaloids together with Flavonoids lignans, steroids, Terpenoids and coumarins have been ident...