Chemopreventive Influence of Kolaviron on 1,2-Dimethylhydrazine Induced Plasma Carbonyl Content and Non-Enzymatic Antioxidant Status in Rat Colon Carcinogenesis
Journal Title: Scholars Academic Journal of Pharmacy - Year 2015, Vol 4, Issue 2
Abstract
Colon cancer is the second leading cause of cancer death worldwide with diet playing a prominent role in disease initiation and progression. We have investigated the chemopreventive efficacy of kolaviron on plasma protein oxidation and non-enzymatic antioxidant status on 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis. Male albino Wistar rats were randomly divided into six groups. Group 1 served as control, animals have access to water and the rodent feeds for 8 weeks plus 1mM EDTA-saline injection subcutaneous (s.c) once a week for 4 weeks. Group 2 rats served as kolaviron (KV) control received 100 mg/kg bodyweight of kolaviron per oral (p.o.) every day. Group 3 served as gallic acid (GA) control, received pellet diet and 50 mg/kg bodyweight of gallic acid p.o. every day. Group 4 served as carcinogen control, received pellet diet and 30 mg/kg bodyweight of 1,2-dimethylhydrazine (DMH) subcutaneous injection once a week for 4 weeks to induce colon carcinogenesis. Group 5 rats received DMH injection and kolaviron 100 mg/kg bodyweight. Group 6 rats received DMH injection and gallic acid 50 mg/kg bodyweight. The results of this experiment shows significant decrease levels of Vitamin C and Total Antioxidant Capacity in plasma of carcinogen exposed groups as compared with control group. Again the levels of carbonyl content and Lipid Hydroperoxide were significantly higher as compared with the control group. Supplementation with kolaviron or gallic acid increases the levels of Vitamin C and Total Antioxidant Capacity but decreases the levels of carbonyl content and Lipid Hydroperoxide significantly as compared with the DMH group. These findings suggest that both kolaviron and gallic acid can significantly reduce the formation of plasma free radicals which are crucial in colon carcinogenesis and effectively modulate the levels of vitamin C and Total Antioxidant Capacity in rats. Keywords: Colon cancer¸ carcinogenic, 1,2-dimethylhydrazine (DMH), Antioxidant activity, kolaviron.
Authors and Affiliations
Eboh A. S, Chuku L. C, Uwakwe A. A
Keywords
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