COMPARATIVE ANALYSIS OF THE GROWTH OF Helicobacter Pylori IN DIFFERENT BLOOD AGAR MEDIUM
Journal Title: Mintage journal of pharmaceutical and medical sciences - Year 2017, Vol 6, Issue 5
Abstract
Objective: Helicobacter pylori are the gram negative pathogenic bacteria mainly associated with gastrointestinal disorders. The growth of the H. pylori has been achieved only by inoculating the clinical specimen and storage in blood agar only. The objective of this research was to compare the growth of H. pylori in different blood agar medium to elucidate whether the medium provided considerable growth of H. pylori in vitro. Methods: H. pylori was inoculated in human, goat, sheep, fish and chicken blood agar plates and incubated in anaerobic under microaerophilic (5% O2, 10% CO2 and 85% N2) conditions at 37°C for 4 days. After successful incubation, significant differences in growth was observed and further confirmed based on colony morphology, Gram staining and biochemical tests. Results: In human blood agar plates, it was observed that creditable colony size and growth of H. pylori. In goat blood also showed more or less equal results same like human blood; whereas minimal growth was observed in chicken, and no growth was observed in sheep and fish blood agar plates. Even though the H. pylori colonies were observed on chicken blood agar plates, the urease test of the colonies are very slow reactive than human and goat blood agar. But other parameters and characterization of goat blood agar are more or less equal like human blood. Conclusion: No previous report observed in the determination of H. pylori growth in different blood culture medium. Thus, this study is suggesting the usage of goat blood as the alternative for the human blood agar for H. pylori isolation and subculturing.
Authors and Affiliations
Sampath Gattu, N. Prabhusaran, k Sridharan, N Kayalvizhi
Keywords
Related Articles
- EP ID EP436124
- DOI -
- Views 131
- Downloads 0
PROCESS OPTIMIZATION AND INVITRO EVALUATION OF RISEDRONATE SODIUM IMMEDIATE RELEASE TABLETS
Objective: Present project aims to process optimize and evaluate 150mg of Risedronate Sodium tablets. Objective of the process optimization plan is to monitor the manufacturing process of Risedronate sodium tablets 150mg...
FORMULATION AND EVALUATION OF FLOATING TABLET OF LABETALOL HYDROCHLORIDE
Objectives: The present study was undertaken to prolong the release of orally administered drug. The objective of the study was to formulate the sustained release floating tablet of labetalol hydrochloride. Experimental...
WATER MEDIATED DIELS-ALDER [4+2] CYCLOADDITION REACTION: A GREEN CHEMICAL APPROACH FOR THE SYNTHESIS OF SOME 2-(6-METHOXY-2-NAPHTHYL)-3-(SUBSTITUTED PHENYL) BICYCLO [2.2.1] HEPTA-5-ENE-2-ONES AND EVALUATION OF BIOLOGICAL ACTIVITIES
Objective: To synthesis some 2-(6-methoxy-2-naphthyl)-3-(substituted phenyl) bicyclo[2.2.1] hepta-5-ene ketones and evaluation of their biological activities. Methods: Green chemical approach of water mediated fly-ash...
ANTIOXIDANT POTENTIAL OF GUNDRUK - AN ETHNIC FOOD OF SIKKIM
Aim: Gundruk is one of the ethnic foods of Sikkim. Ethnic group in Sikkim believe that gundruk is a good appetizer and keeps their body fit and promotes health. In view of this belief it was thought worthwhile to evaluat...
ANTIMICROBIAL POTENT OXAZINE-2-AMINE DERIVATIVES
Objective: To synthesize thirteen effective microbial potent oxazine-2-amine derivatives and evaluate the antimicrobial activities of these compounds. Material and methods: The solvent-free solid fly-ash: H2SO4 catalyzed...