COMPARATIVE BOTANICAL AND GENETIC CHARACTERIZATION OF CERTAIN SOLANUM SPECIES GROWN IN EGYPT
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objective: Urgent need for proper identification and characterization has emerged for some Solanum species as their toxicity to humans and animals ranges from mildly irritating to fatal. The objective of this work was targeted towards discrimination between Solanum seaforthianum Andrews and Solanum macrocarpon L.Methods: For establishment of different botanical and genetic criteria, this study presents a comparative investigation of the botanical features of the roots, stems and leaves of both plants through microscopical investigation of the prepared entire, transverse sections and powdered forms of different organs of both plants under study. Furthermore, the DNA of both plants was extracted from leaf samples and Random Amplified polymorphic DNA (RAPD) analysis using ten primers of arbitrary sequences.Results: Comparative botanical characters of different organs were identified. On the other hand a total 101 fragments were generated in S. macrocarpon while 105 fragments were generated in S. seaforthianum. Where the highest degree of similarities (70%) was recorded using primer B16 therefore could be used as an indicator for obtaining genetic markers, followed by 65.38% for Z13 and the lowest degree of similarity (38.1%) was recorded using primer O14 which could be used to discriminate between the two Solanum species depending on their low values of similarity coefficients and high level of polymorphism.Conclusion: For the present study, macro and micro-morphological characters, as well as, DNA fingerprinting can be considered as the identifying parameters to authenticate and differentiate between the two plants under study.Â
Authors and Affiliations
Muhammad A. Alsherbiny, Shahira M. Ezzat, Fatma S. Elsakhawy, Mostafa A. Abdel-kawy
Keywords
Related Articles
- EP ID EP578300
- DOI -
- Views 93
- Downloads 0
ONLINE VISIBILITY OF PHARMACY RESEARCH IN TANZANIA: A SCIENTOMETRIC STUDY
Objective: This scientometric analysis was carried out to map the online visibility of pharmacy research at Muhimbili University of Health and Allied Sciences (MUHAS) from 1981 to 2016.Methods: Publish or Perish software...
REVIEW ON PHORBOL ESTER DEGRADATION OF JATROPHA SEED CAKE FOR ITS USE AS ANIMAL FEED
Jatropha curcas is an oil-seed plant with good adaptability to grow in unfavourable conditions like infertile soil with scanty rainfall. It had been exploited for the extraction of oil for bio-diesel. The compressed seed...
INHIBITION OF CANDIDA ALBICANS AND STREPTOCOCCUS MUTANS WITH DATURA LEAF AND SEED EXTRACTS
Objectives: Â Existing antimicrobial compounds are of a limited effectiveness. Therefore, the antimicrobial activity of Datura stramonium extracts was evaluated against two important human pathogens; Candida albicans and...
LIBIDIBIA FERREA (MART. EX TUL) L. P. QUEIROZ VAR. FERREA: PHARMACOLOGICAL, PHYTOCHEMICAL AND BOTANICAL ASPECTS
Libidibia ferrea, popularly known as “pau ferro†or “jucáâ€, is an important medicinal plant employed for the treatment of various ailments in the North and Northeast regions of Brazil, including anemia, inflamma...
SOME NATURAL PRODUCTS AND THEIR SECONDARY METABOLITES ATTRIBUTED TOWARDS DIABETIC CURE: A REVIEW
Diabetes is one of the major health and development challenges of the 21st century. According to the International Diabetes Federation, there are currently more than 371 million people living with diabetes and another 28...