Comparison of vegetative buds formation in two date palm cultivars, Medjool and Mazafati through direct organogenesis
Journal Title: International Journal of Farming and Allied Sciences - Year 2015, Vol 4, Issue 6
Abstract
Micro-propagation through direct organogenesis is widely used for rapid propagation of superior cultivars of date palm.In the present study, the shoot tip explants were cultured on Murashige and Skoog (1962) (MS) mediasupplemented with different plant growth regulator (PGR) treatmentsfor inducing buds regeneration. The experiment was set up in a factorial based withtwo factors.Factor A was PGR treatments in four levels (1- 2iP: 1mg/L, BAP: 1mg/L, 2- 2iP: 0.5mg/L, BAP: 0.5mg/L, 3- 2iP: 0.2mg/L, BAP: 0.2mg/L, 4- 2iP:0.1mg/L, BAP:0.1mg/L) and factor B was two date palm cultivars. The results showed that the PGR treatments had significant effects on vegetative buds formation and the best treatment was 0.5mg/L1-naphthylacetic acid, 0.5mg/L2-naphthyloxyacetic acid,1mg/L N6-(2- isopentyl)adenine, 1mg/L6-benzylaminopurine. Also, significant difference observed between cultivars and Medjool produced more vegetative buds (2.62) thanMazafati cultivar.
Authors and Affiliations
Marjan Roshanfekr Rad, Reza Zarghami, Hassan Hassani and Hedayat Zakizadeh
Keywords
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