CYTOTOXICITY STUDY OF ANTIDIABETIC PLANTS ON NEUROBLASTOMA CELLS CULTURED AT NORMAL AND HIGH GLUCOSE LEVEL
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objective: In diabetes, chronic hyperglycemia causes damage (glucose toxicity) on some cells leading to micro and macro vascular complications. The aim of this study was to investigate the effect of antidiabetic plants extracts in high glucose concentration in vitro.Methods: Phyllanthus amarus (whole plant), Vitex doniana (leaves), Tectona grandis (leaves and trunk bark) and Plumeria alba (roots) hydroalcoholic extract (at the concentrations of 6.25, 25, 75, 125, 250 and 500 µg/ml) were tested for their possible cytotoxicity using the 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on neuroblastoma cells lines in standard condition (extract alone) and high glucose concentration (extract+50 mM glucose).Results: At concentrations of 6.25 and 25µg/ml, T. grandis bark and leaves and P. amarus induced a significant decrease (p<0.01; p<0.001) on cell viability as compared to controls. The decrease on cell viability was very pronounced in the presence of the extracts plus glucose 50 mM. P. amarus extract becomes increasingly toxic as the concentration of extract increased in the presence of glucose. With P. amarus at 125 µg/ml and glucose at 50 mM, there is no more viable cells in the medium. By contrast, T. grandis bark extract induced a significant reduction of the cytotoxicity in the presence of glucose compared to standard condition.Conclusion: It appears that, only hydroalcoholic extract of T. grandis bark possesses neuroprotective activity in high glucose concentration.Â
Authors and Affiliations
Povi Lawson Evi, Aboudoulatif Diallo, Batomayena Bakoma, Serge Moukha, Kwashie Eklu GadegbÉku, Kodjo Aklikokou, Edmond Creppy, Messanvi Gbeassor
Keywords
Related Articles
- EP ID EP578132
- DOI -
- Views 48
- Downloads 0
ZERO, FIRST, SECOND ORDER DERIVATIVE AND AREA UNDER CURVE SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF CEFIXIME TRIHYDRATE IN PHARMACEUTICAL FORMULATION
Objective: A simple, accurate, precise and specific zero, first, second order and area under curve spectrophotometric methods has been developed for determination of Cefixime Trihydrate in its tablet dosage form by using...
KINETICS AND MODELING OF TANNASE PRODUCTION USING ASPERGILLUS FOETIDUS IN BATCH FERMENTATION
Objective: To produce tannase enzyme using Aspergillus foetidus with red gram husk as substrate in batch fermentor and investigate the suitable un structured kinetic model for the system.Methods: The present study was do...
SCREENING OF PHYTO-CHEMICALS, TLC PROFILING, TOTAL FLAVONOID AND PHENOLICS CONTENT, ANTI-OXIDANT ACTIVITY AND ANTI-MICROBIAL ACTIVITY OF FICUS BENGHALENSIS LINN AND FICUS RELIGIOSA LINN LATEX
Objective: Screening for the presence of phyto-chemicals present in the plant extracts by qualitative assay along with Thin Layer Chromatography (TLC) investigation followed by determining the total flavonoids and phenol...
CHEMICAL CONSTITUENTS, IN VITRO ANTIOXIDANT ACTIVITY, ORAL ACUTE TOXICITY AND LD50 DETERMINATION OF MORINGA OLEIFERA LEAVES
Objective: The objective of this study was undertaken to estimate the total phenolic contents (TPCs), in vitro antioxidant of different solvent extracts of M. oleifera leaves, oral acute toxicity and LD50 determination o...
BIOMECHANICAL, BIOCHEMICAL AND HISTOLOGICAL EVIDENCES FOR WOUND HEALING PROPERTIES OF INDIAN TRADITIONAL MEDICINES
Objective: Ayurveda, India's traditional medicinal system is a rich source of natural remedies, frequently used as home and folk medicine in wound healing due to easy availability and affordability. Honey, Ghee and roots...