Demonstration of Specific Marker Proteins, Determining Spermatozoa Fertility, and of Proteins, Common for Sperm and Other Cellular Types (Stem/Progenitor, Malignant and Differentiated Normal Cells)
Journal Title: Journal of Clinical and Medical Research - Year 2020, Vol 2, Issue 3
Abstract
The condensation of nuclear chromatin in mature differentiated male germ cells is an important morphological marker about their fertilization capability or male fertility, respectively. Spermatozoa with decondensed chromatin possess a sharply reduced ability to fertilize the egg and they are even responsible for the early abnormal development of the fetus. The aim of the current study is directed to demonstration of specific proteins/markers (cytoplasmic and/or nuclear DNPs/RNPs), determining the fertility of male germ cells, as well as of proteins, which are common/specific for sperm and other cell types (such as embryonic and adult stem/progenitor cells, malignant HeLa cells, etc.) at different stages of their development. Combining different techniques for assay could give a possibility about more precise identification of the changes, and inter-molecular (DNP/RNP and of protein-protein) interactions in the composition of complex cytoplasmic structures, on intra- and extra- cellular levels. The results obtained could be helpful to establish the diagnosis as well as to the application of adequate therapeutic strategy and/or of appropriate techniques for in vitro-fertilization (assisted reproductive technology) in cases of male infertility. The current article demonstrates for specific marker proteins.
Authors and Affiliations
Iliana N Ilieva*, Iskra V Sainova and Bojcho Nikolov
Keywords
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- EP ID EP708397
- DOI https://doi.org/10.37191/Mapsci-2582-4333-2(3)-034
- Views 70
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