Detection and Assay of Antimycobacterial AgentIsoniazid Utilizing Isocratic High PerformanceLiquid Chromatography
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 5
Abstract
Isoniazid was assayed by HPLC, utilizing a reversed-phase C-18 column with eluent solvent (5.3% ethanol, 93.7% water, 1% acetic acid). Samples for analysis were prepared in distilled water as the solvent. Detection of isoniazid was accomplished at 265 nm, which showed highest sensitivity for isoniazid . Column pump pressure approximately 2100 psig, rise time 0.1 with flow rate 1.0 mL/minute. Elution of isoniazid occurred at 2.2 minutes. Limit of detection (LOD) of isoniazid was 1.172 x 10-5 molar, but with detection going as high as 1.460 x 10-3 molar which provides a detection range of 125x. The limit of quantitation (LOQ) is 3.905 x 10-5 molar. Reverse phase isocratic conditions is shown to be effective for determination of isoniazid in aqueous based samples. The standard curve is highly linear reaching as high as 1.460 x 10-3 molar (y = 50570789.86 x), having coefficient of determination (R2 = 0.9916) with very strong positive correlation coefficient (r= 0.9958). The percent recovery of drug ranged from 96% to 104%. Utilizing reversed phase column with isocratic solvent conditions (ethanol, acetic acid, and water in column solvent), is effective for determination of isoniazid.
Authors and Affiliations
Ronald Bartzatt 1 ,, Purnima Gajmer1, Mai Han Cassandra Nguyen1 ,, Alexandra My-Hanh Tran1
Keywords
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