Detection of Dengue Virus in Department of Bolivar, Colombia, 2012-2013, Real-Time PCR
Journal Title: JOURNAL OF ADVANCES IN BIOTECHNOLOGY - Year 2013, Vol 3, Issue 3
Abstract
Objective. To assess the presence of RNA from dengue virus by Real Time Polymerase Chain Reaction with Reverse Transcriptase (qRT-PCR), in anti-dengue IgM seronegative serum samples obtained from symptomatic patients with less than 5 days of fever, in the department of Bolivar, Colombia.Materials and Methods. Cross sectional descriptive study, where 50 anti-dengue IgM seronegative serum samples were analyzed by qRT-PCR. In addition, we reviewed the epidemiological charts of each patient in order to collect sociodemographic and blood count (CBC) data.Results. DENV-3 RNA was detected in seven (14Â %) serum samples. The most common clinical manifestations were: fever (90Â %; 45/50), headache (88Â %; 44/50) and myalgia (74Â %; 37/50). Regarding the CBC, the most significant findings were: thrombocytopenia (60Â %; 30/50; 73.3% were men), and leukopenia (50Â %; 25/50; 60% were men).Conclusion. Detection and typing of DENV genome by qRT-PCR could be considered a diagnostic tool for Dengue in anti-dengue IgM seronegative serum samples, especially, within four days after the onset of the signs of the disease.
Authors and Affiliations
DORIS ESTHER Gómez Camargo, Yina Paola García Toscano, MARGARITA MARIA OCHOA, Raimundo Castro, Erwin Camacho, Pedro Blanco Tuirán
Keywords
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- EP ID EP653852
- DOI 10.24297/jbt.v3i3.1668
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