Determination of the minimum inhibitory concentration (MCI) of Hygromycin B for its use as a selection agent in the transformation of Mycosphaerella fijiensis
Journal Title: Biotecnología Vegetal - Year 2001, Vol 1, Issue 2
Abstract
The Black sigatoka can be considered from the economic point of view the most serious disease in the gender Musa. At present the use of new biotechnological techniques applied to the study of the host-pathogen interation is glimpsed as an important tool in the future improvement of the commercial cultivars of the banana and plantain. In this study, we tested diferents concentration of Hygromycin B against an isolate of M. fijiensis (CCIBP-1) by the agar dilution method. It was proved that Hygromycin B was able to inhibit the growth of the M. fijiensis starting from 0.5 mg.l -1. This value was taken as the minimum inhibitory concentration, that’s why it could be used like selective agent in the transformation studies of Mycosphaerella complex. By another hands it was demostrated the value of the previous protocol of the Minimum Inhibitory concentration for making evaluations of different fungus pathogens against antimicribial substance.
Authors and Affiliations
Mileidy Cruz-Martín, Mayra Acosta-Suárez, Edrey A. Rodríguez, Michel Leiva, Yelenys Alvarado Capó
Keywords
Related Articles
- EP ID EP697721
- DOI -
- Views 49
- Downloads 0
In vitro phosphate solubilization by Aspergillus and Penicillium strains and coffee plants growth promotion
The availability of phosphorus in the soil for the growth of plants can be increased by the action of phosphate solubilizing microorganisms. The objective of this work was to determine the potential of Aspergillus and Pe...
Tissue culture and genetic transformation in Glycine max (L.) Merrill
This work is a brief overview on the background of in vitro tissue culture and genetic transformation of soybean, as well as a brief introduction to the origin, distribution and importance of the crop. It is also aimed...
Effect of the height of Coffea arabica cv. Caturra rojo J-884 plants obtained by somatic embryogenesis in the ex vitro adaptation
The asexual propagation through somatic embryogenesis has been developed in different coffee cultivars (Coffea arabica L. and Coffea canephora Pierre ex Froehner). This investigation was carried out with the objective...
Response of Phaseolus vulgaris L. cultivars under induced conditions of heat stress
The production of common bean (Phaseolus vulgaris L.) is limited by diverse stressful conditions, amongst them high temperatures. Therefore, obtaining tolerant bean cultivars without affecting their yield is a purpose...
Characterization of leaf area of Agave fourcroydes Lem. plants obtained from asexual propagation
The henequen (Agave fourcroydes Lem.) is a crop of great economic importance. This study was aimed to characterize the leaf surface of henequen plants variety ‘Sac Ki’ obtained by asexual propagation methods. In vitro...