Abstract

Developing a single analytical method for the estimation of individual drugs is very challenging, due to the formation of drug-drug and drug-excipient interactions. The present study demonstrates the applicability of thechromatographic method to develop a new, sensitive, single HPLC method for the simultaneous quantitative determination of three antiviral agents in thefixed pharmaceutical dosage form. Chromatographic separation of the three antiviral drugs was achieved by using a gradient elution at a flow rate of 1.5 mL/min on Inertsil ODS 3V C18 column (250m×4.6mm, 5μm particle size, 100Å pore size) at ambient temperature. Mobile phase A of the gradient solvent system was KH2PO4 (0.03M) in 1000 ml of water and by adjusting the pH to 3.2 with dilute ortho-phosphoric acid and mobile phase B was Methanol and water in the ratio of 85:15 v/v. UV detection at 265 nm was employed to monitor the analytes. A linear response was observed for Paritaprevir over the concentration range 30–90 μg/ mL, 20-60 μg/ mLof Ritonavir and5-15μg/ mL of Ombitasvir.

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