Development and validation of new RP-HPLC method for simultaneous estimation of drug ceftriaxone and vancomycin in tablet dosage form
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2016, Vol 4, Issue 4
Abstract
A simple, economic, accurate and precise reverse phase high performance liquid chromatography method for analysis of Ceftriaxone and Vancomycin was developed and validated according to ICH guidelines. The quantification of the drug was carried out using PDA (photodiode array) detector. Column in isocratic mode, with mobile phase Acetonitral : Methanol in ratio of 90:10 was used. The flow rate was 1.0 ml/min and effluent was monitored at 241nm.the retention times were 3.884 and 4.470 min for Ceftriaxone and Vancomycin respectively. The injection volume was 20µl.as per ICH guidelines the method was validated and the method was found to be linear in the range of 20-80µg/ml for Ceftriaxone and Vancomycin. Percent recovery studies of Ceftriaxone and Vancomycin 98.00%and101.50%. The limit of detection and quantification was found to be 0.177&0.537µg/ml Ceftriaxone and 0.055&0.167µg/ml for Vancomycin .the values of precession and robustness lie within the acceptance limit. Thus the proposed method can be successfully applied for simultaneous determination of Ceftriaxone and Vancomycin in routine analysis work.
Authors and Affiliations
Paleti Mamatha
Keywords
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