DEVELOPMENT OF AMPEROMETRIC BIOSENSOR SYSTEM FOR SIMULTANEOUS DETERMINATION OF PYRUVATE AND LACTATE
Journal Title: Сенсорна електроніка і мікросистемні технології - Year 2017, Vol 14, Issue 4
Abstract
This article describes the development of a biosensor system consisting of two monobiosensors based on pyruvate and lactate oxidases for determination pyruvate and lactate, respectively. For the enzymes immobilization in the bioselective membrane, the method of capturing into PVA-SbQ polymer on the surface of amperometric transducer was used. Platinum disk electrodes served as the transducers. The processes of production of both monobiosensors as well as the conditions of their operation were identical, which makes their integration into a single system possible. The conditions of enzymes immobilization and parameters of buffer solution (pH, buffer capacity, ionic strength) were optimized and universalized for simultaneous operation of biosensors. The cross influence of substrates and cofactors on enzymes was examined. The biosensor system is characterized by high degree of operational stability and reproducibility to responses to pyruvate and lactate. The obtained analytical characteristics of the biosensor system testify to the possibility of efficient usage for lactate and pyruvate analyses in real biological fluids.
Authors and Affiliations
Ya. V. Topolnikova, D. V. Knyzhnykova, I. S. Kucherenko, S. V. Dzyadevych, O. O. Soldatkin
Keywords
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- EP ID EP398737
- DOI 10.18524/1815-7459.2017.4.119596
- Views 63
- Downloads 0
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