DEVELOPMENT OF MATHEMATICAL MODEL OF POTENTIOMETRIC BIOSENSOR BASED ON THE REVERSIBLE ACETYLCHOLINESTERASE INHIBITION FOR AFLATOXIN B1 DETERMINATION
Journal Title: Сенсорна електроніка і мікросистемні технології - Year 2016, Vol 13, Issue 1
Abstract
This work presents a mathematical model of a potentiometric biosensor based on the reversible acetylcholinesterase inhibition for aflatoxin B1 determination. An actual potentiometric biosensor based on immobilized acetylcholinesterase was used in this work for comparison with a mathematical model and validation. The mathematical model is described by a system of rate equations, presenting the dynamics of biochemical reactions in the biosensor. Each equation describes concentrations of the enzyme, substrate, inhibitor, product, or concentrations of enzyme-substrate, enzyme- inhibitor, enzyme-substrate- inhibitor complexes as a function of time. The system is solved numerically using Wolfram Mathematica software. Initial concentration of the enzyme, substrate and inhibitor act as boundary conditions for the system of rate equations. The concentrations have been calculated from the experimental conditions: 2 × 10-5 M acetylcholinesterase, 4 × 10-3 M acetylcholine chloride, and 0.2 mg / ml of aflatoxin B1 for the enzyme, substrate, and inhibitor respectively). The rate constants have been chosen to fit the experimental response. It is shown that the kinetic model developed allows to reproduce the performance of a real potentiometric biosensor
Authors and Affiliations
K. Stepurska, S. Dzyadevych
Keywords
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- EP ID EP396849
- DOI 10.18524/1815-7459.2016.1.70328
- Views 85
- Downloads 0
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