Development of Dextrose Base Formulation of Bacillus Subtilis and Its Evaluation as A Biocontrol Agent against Fusarium Oxysporum F.Sp.Ricini
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 5
Abstract
In the present study; B. subtilis NSPLKMB01 has been isolated from local soil of Jalgaon district, Maharashtra, India, identified as a B. subtilis strain NAIMCC-B-02000, by 16S rRNA gene sequencing and developed formulation, using simple sugar dextrose anhydrous, as a carrier. Then bio-efficacy of the formulation was tested in randomized block design, against castor wilt causing agents Fusarium oxysporum f. sp. ricini, in pot trial through seed treatment. The results of a pot trial revealed that, treatment (T5), dextrose base Bacillus subtilis NSPLKMB01, 10g per kg of seed, gave highest disease control which was (73.41%) followed by chemical fungicide, treatment (T6) thiram (70.08%) and treatment (T1) talc base formulation of Bacillus subtilis NSPLKMB01 (70.14%). Similarly, maximum seed germination also recorded in treatment (T5), which was (94.10%), followed by chemical fungicide thiram (T6) and talc base formulation of Bacillus subtilis (T1), 91.09% and 83.92%, respectively.
Authors and Affiliations
Valmik M. Patil, Kishor R. Patole, Mohan S. Paprikar, Jaysingh C. Rajput
Keywords
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