Differential response of three Phaseolus vulgaris L. cultivars to in vitro water stress induced by PEG-6000
Journal Title: Biotecnología Vegetal - Year 2020, Vol 20, Issue 4
Abstract
The growth and agricultural yield of common beans (Phaseolus vulgaris L.) is affected, among other factors, by limitations in the availability of water. For this reason, there are working on the search for cultivars tolerant to that abiotic stress. The objective of the research was to determine the effect of different concentrations of PEG-6000 on the in vitro seeds germination of three cultivars of common bean (ʹIca Pijaoʹ, ʹBAT-93ʹ and ʹTío Canela-75ʹ) with different responses to water stress. PEG-6000 was used as an in vitro stress-inducing agent at 10, 12 and 14% (m / v) in the liquid culture medium. Deionized water was used as a control. Seeds of each cultivar were placed on a filter paper located in the shape of an M inside the test tube containing the corresponding solution. After three days of culture, the number of germinated seeds was quantified and the percentage of germination was calculated. Subsequently, at seven days the length of the root, hypocotyl and epicotyl, respectively, was measured. In the three cultivars, water stress affected the in vitro germination of the seeds in each of the treatments. There was a significant decrease in the values of the variables evaluated with respect to the control. Specifically, the growth of epicotyl was inhibited. The response to water stress in vitro induced with PEG 600 in each cultivar was specific and differential. The results contribute to the development of early selection strategies in the genetic improvement program of this crop.
Authors and Affiliations
Milady F Mendoza-Rodríguez, Amanda Martirena-Ramírez, Luis E Rojas, Damaris Torres, Samuel Hernández, Leonardo Rivero
Keywords
Related Articles
- EP ID EP697695
- DOI -
- Views 86
- Downloads 0
Effect of sucrose concentration and growth regulators in the in vitro tuberization of Dioscorea alata L. variety Cartagena
Among the species of Dioscorea which tubers are used as human food, D. alata L. is one of the most productive and important economically. In this work the effect of sucrose (0, 2, 4, 6, 8,10%) and growth regulators conce...
Aspects related to the physical environment and characterization molecular structure of somatic embryogenesis
In the current work, a review on the influence of the in vitro environment in the morphogenetic process of the somatic embryogenesis is carry out, specifically the effect of certain gases accumulated in the gas phase of...
In vitro germination of Dendrocalamus strictus (Rosb.) Nees seeds
The availability of D. strictus seeds is affected by the length and erratic flowering cycle of the species. These seeds also retain their germination only for short periods of time. The tissue culture offers a fast and...
Callus formation of sugar cane var. ‘SP 70-1284’ in liquid culture medium from field explants
Explants from immature sections of young leaves in the stems of the variety of sugarcane ‘SP 70-1284’, were taken directly of field whit the objets of study the capacity for organogenic callus formation. The effect of t...
Callus formation inAnthurium magnificum Linden
Anthurium magnificum Linden is a very popular ornamental plant due to its beautiful foliage.In this species, traditional propagation methods are not practical for its large-scale production,and there are no studies in th...