EDTA induced morphological changes in the cells of peripheral blood smear
Journal Title: INTERNATIONAL JOURNAL OF RECENT TRENDS IN SCIENCE AND TECHNOLOGY - Year 2017, Vol 22, Issue 2
Abstract
Microscopic evaluation of peripheral blood smear [PBS] is a very important investigation. PBS is often prepared using blood samples collected in an anticoagulant. EDTA is an anticoagulant of choice for various laboratory analyses. However it can cause morphological changes in blood cells. These artefacts can lead to misinterpretation of PBS. The present study was undertaken to identify EDTA induced artefacts in PBS. Blood samples were collected directly in EDTA, mixed thoroughly and PBSs were made immediately and at 1hr, 2hr, 4hr and 6hr after storing the samples at room temperature. Finger prick smears prepared simultaneously from same patients served as controls. Artefacts were noticed initially at 2hr and marked changes were observed at 6hr. It is important to make PBS from EDTA anticoagulated blood samples within 1hr of blood collection to avoid morphological changes in blood cells however it is highly advisable to prepare PBS immediately after sample collection to ensure perfect interpretation of PBS.
Authors and Affiliations
Jayashree Sathe, Sanjay Pramanik, Rajesh Jambhulkar
Keywords
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