Effect of 2,4-D concentration in somatic embryos formation of cuban soybeans varieties
Journal Title: Biotecnología Vegetal - Year 2012, Vol 12, Issue 2
Abstract
Developing new breeding programs of soybean crops needs an efficient systems for plants regeneration. Eventhough due this is impossible taking into account its genotype dependent condition. This study aimed to: determine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration in the formation of somatic embryos in soybean Cuban varieties (Incasoy-1, Incasoy-24, Incasoy-27 and Incasoy-35). Immature cotyledons were used as plant material. Three treatments were established according to the concentrations of 2,4-D (30, 40 and 50 mg l -1 ). The number of somatic embryos per immature cotyledon were evaluated after 30 days. The necessary concentration of 2,4-D to obtain the mayor formation of embryos depended on the genotypes (30 mg l -1 for Incasoy-24; 40 mg l -1 for Incasoy-27 and Incasoy-35; 40 and 50 mg l -1 for Incasoy-1). Depending on the results obtained the concentration 40 mg l -1 for the cuban varieties was selected The Incasoy-24 was the exception since it responded better with a concentration of 30 mg l -1 . These results favor the development of new programs for genetic breedig of soybeans in Cuba.
Authors and Affiliations
Idalmis Bermúdez-Caraballoso , Teresa Del Socorro Blanco , Jorge Pérez-Pérez , Lourdes R. García , Novisel Veitía , Raúl Collado , Damaris Torres , Carlos Romero
Keywords
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