EFFECT OF COMBINATION OF TWO PLANT EXTRACTS ON DIABETES MELLITUS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 4
Abstract
Objective: To investigate the anti-diabetic activity of combined ethanolic extracts (1:1mixture) of dry leaves of Syzygium cumini and Psidium guajava belonging to the family Myrtaceae as well as to compare the anti-diabetic activity of these plants by in vitro methods.Methods: In vitro glucose uptake assay was performed on cultured L6 cell lines (rat myoblast cell line) and estimated the glucose uptake using high sensitivity glucose oxidase kit. In vitro alpha amylase inhibitory assay was performed on porcine alpha amylase and the absorbance was measured at 540 nm using a microplate reader. Acarbose was used as the standard in both the methods.Results: At a concentration of 100µg/ml the percentage glucose uptake by the combined ethanolic extract (1:1 mixture) of Syzygium cumini and Psidium guajava leaves was 43.95 while for acarbose the corresponding value was 51.71. At 100 μg/ml the percentage of glucose uptake by Syzygium cumini and Psidium guajava was 27.62 and 22.17 respectively. The percentage inhibition of alpha amylase by the combined ethanolic extract (1:1 mixture) of Syzygium cumini and Psidium guajava leaves at a concentration of 1000 µg/ml was 36.51 and it was 29.26 for Syzygium cumini and 23.43 for Psidium guajava. For acarbose the percentage inhibition of alpha amylase was 73.82 at the concentration of 1000 µg/ml.Conclusion: The combined extract of the leaves of the plants selected was found to be more effective than individual plant extracts against diabetes. The percentage glucose uptake of the combined extract was found to be closer to that of the standard drug acarbose. On comparison of two plants Syzygium cumini was found to be more active against diabetes than Psidium guajava. As the 1:1 mixture of the ethanolic extract is found to be more active, the combination of the two plants can be used to formulate drugs for treating diabetes.
Authors and Affiliations
Jose Deepa, N. A. Aleykutty, Harindran Jyoti
Keywords
Related Articles
- EP ID EP567523
- DOI 10.22159/ijpps.2018v10i4.24100
- Views 80
- Downloads 0
DEVELOPMENT AND IN-VITRO EVALUATION OF QUANTUM DOTS AS A CARRIER FOR DELIVERY OF 5-FLUOROURACIL
Objective: The present study was aimed to develop and evaluate quantum dots (QDs) as a carrier for delivery of 5-fluorouracil (5-FU).Methods: This research work includes a synthesis, characterization and in vitro study o...
A CASE REPORT: DRUG INTERACTION BETWEEN LINEZOLID AND DOPAMINE
Linezolid is a weak, non-selective inhibitor of monoamine oxidase (MAO). It can inhibit the breakdown of the tyramine by MAO in the gut and can also potentiate the effect of tyramine at nerve endings, thereby causing an...
COMPARATIVE STUDY OF ANTIOXIDANT AND ANTICANCER ACTIVITY OF ALPINIA CALCARATA AND ALPINIA GALANGA
Objective: The present study was to determine in vitro antioxidant and anticancer activity of Alpinia calcarata and Alpinia galanga.Methods: The phytochemical screening of rhizome of aqueous extract of Alpinia calcarata...
FORMULATION AND IN-VITRO EVALUATION OF MOXIFLOXACIN OCULAR INSERTS
Objectives: Most ocular diseases are treated with topical applications of eye drops. After instillation of an eye drop, typically less than 5 % of the applied drug penetrates the cornea and reaches intraocular tissues, w...
DEVELOPMENT AND OPTIMIZATION OF ORODISPERSIBLE TABLETS OF CARVEDILOL BY COMBINATION OF SUPER-DISINTEGRANTS ADDITION AND SUBLIMATION TECHNIQUES
Objective: Objective of the present research work was to prepare orodispersible tablets of carvedilol (CDL) for dysphagic patients.Methods: Carvedilol, an anti-hypertensive drug, was chosen as a model drug in this study....