Effect of harmones on callus induction in Maize (Zea mays L.)
Journal Title: Journal of Applied and Natural Science - Year 2018, Vol 10, Issue 1
Abstract
Callus induction from explants is a critical process in regeneration, micropropagation and transformation of maize (Zea mays L.) plants. Formation of callus from plant tissues on culture is affected by several factors. This study revealed to establish the effect of genotype, source of explants and auxin concentration on callus induction from five genotypes UMI 757 (G1), UMI 615 (G2), UMI 112 (G3), UMI 285 (G4) and CO 1 (G5) and one hybrid CO H (M) 5 (G6). Callus induction of the six maize varieties was investigated using immature embryos (E1), leaf bits (E2), root tips (E3), hypocotyls (E4) and seeds (E5) as explants with different concentrations of hormones. In this study, immature embryo was taken from 10 to 12 days after pollination (DAP) to get maximum response. The highest percentage of callus induction was observed (99.10) in immature embryo culture and seed culture gave the highest percentage of rhizogenic callus formation when compare to immature embryo. Among the genotypes tested, CO H (M) 5 recorded the highest callus induction percentage on (2D2K2) medium composition.
Authors and Affiliations
N. Malini, C. R. Anandkumar, R. Gnanam, S. Hari Ramakrishnan
Keywords
Related Articles
- EP ID EP287184
- DOI 10.31018/jans.v10i1.1605
- Views 118
- Downloads 0
Gene action and component of genetic variance analysis in the thermo sensitive genetic male sterile (TGMS) line in rice (Oryza sativa L.)
The field experiment conducted with eighteen TGMS lines, seventy two F1’s and four checks in randomized complete block design with three replications at the Norman E. Borlaug Crop Research Centre of Govind Ballabh Pant U...
Effect of dietary zinc on the growth and metabolic enzyme activities of golden mahseer (Tor putitora) fry
The golden mahseer (Tor putitora) is an important and high valued sport and food fish of national and international importance. Therefore for promotion of golden mahseer, proper mineral supplementation in early developme...
Effect of processing on physico-chemical and functional properties of flours from cluster or guar bean (Cyamopsis tetragonoloba) varieties
Guar bean (Cyamopsis tetragonoloba) offers a potential source of vegetable protein and fibre, however, its utilization in food is yet to be explored. Along with the nutritional value, its effect on other properties of fo...
Isolation and characterization of flavone di-glucoside and acetoxyxanthone from the flowers of Bombex ceiba
In present study two chemical constituents viz. Flavon 5,4’ dimethoxy 8 methyl 7-O- β -D glucopyranoside-5’- β -D-glucopyranoside and xanthone 3-acetoxy-1-hydroxy-6-methoxy8-O– β –D-glucopyranosyl-(1 → 3)- α –L- rhamnopy...
Characterization and categorization of Indian mustard genotypes for agro-morphological traits
A total of sixty genotypes and germplasm lines were evaluated and characterized for 14 quantitative and 8 qualitative traits in Brassica juncea. Observations were recorded on the basis of scores given in the DUS descript...