ENHANCED PRODUCTION OF PSORALEN THROUGH ELICITORS TREATMENT IN ADVENTITIOUS ROOT CULTURE OF PSORALEA CORYLIFOLIA L.
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 1
Abstract
Objective: The present study aimed to determine the effect of two elicitors namely Methyl Jasmonate (MeJ) and Salicylic acid (SA) on adventitious root production of Psoralea corylifolia L. as the elicitors and at different concentrations.Methods: Adventitious roots of P. corylifolia L. were treated with different concentration of elicitors such as MeJ (10, 20, 30 and 40 µM/l) and SA (50, 100, 150 and 200 µM/l) to enhance the psoralen contents. After the treatment, psoralen content was analyzed through the analytical HPLC experiments (Methanol: Water (50:50) at 0.8 ml/min-1 flow rate and the injection volume as 20 µl).Results: MeJ and SA treatments at the concentrations of 30 µM/l and 150 µM/l respectively were found to increase the net wet weight of adventitious root production to 8 fold at 8 hours of elicitor treatment periods. Further, the quantity of psoralen was determined from the elicitors (MeJ and SA) treated roots and mother plant through the analytical HPLC experiments in order to estimate the psoralen content and it was found as 3.73 mg/ml, 0.015 mg/ml and 0.56 mg/ml respectively.Conclusion: In the present study we achieved enhanced production of psoralen through abiotic elicitors (MeJ and SA) treatment and concluded that the MeJ at 30 µM/l concentration shows significant enhancement of psoralen production.Â
Authors and Affiliations
G Siva, S. Sivakumar, G. Premkumar1, T. senthil Kumarn . Jayabalan
Keywords
Related Articles
- EP ID EP579174
- DOI -
- Views 62
- Downloads 0
SYNTHESIS, CHARACTERIZATION AND ANTIMICROBIAL STUDY OF SOME NOVEL FLUORINE BASED 2-AMINOTHIAZOLES
Objectives: To synthesize, characterize and evaluate antimicrobial properties of some novel fluorine based 2-aminothiazoles.Methods: The syntheses of some novel fluorine based 2-aminothiazoles have been described by usin...
IN VITRO STUDY OF THE CONTRACEPTIVE SPERMICIDAL ACTIVITY OF ENSETE SUPERBUM ON HUMAN SPERM
Objective: The present study was conducted to determine the spermicidal and contraceptive efficacy of ethanolic extract of Ensete superbum seeds on human sperm in vitro.Methods: The hypo-osmotic swelling (HOS) and the sp...
POTENTIAL HEALING EFFECTS OF HIBISCUS SABDARIFFA L. FLOWERS ON ARTHRITIS
Objective: The present study has been focused to assess the anti-inflammatory activity and healing effects of the aqueous extract of Hibiscus sabdariffa L. Flowers on induced arthritis in mice and compare it with meloxic...
A STUDY EVALUATING APPROPRIATENESS OF UTILIZATION PATTERN OF ANTIMICROBIALS IN PATIENTS ADMITTED IN MEDICAL INTENSIVE CARE UNIT OF TERTIARY CARE TEACHING RURAL HOSPITAL
Objectives: To evaluate the appropriateness of utilization pattern of antimicrobials in the intensive care unit of a tertiary care teaching rural hospital.Methods: This prospective and observational study was carried out...
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF ORNIDAZOLE IN TABLET DOSAGE FORM BY ZERO ORDER UV SPECTROSCOPY
Objective: To develop and validate two simple and cost effective spectrophotometric methods for the determination of Ornidazole (OND) in pure form and in pharmaceutical tablet dosage form.Methods: Single point standardiz...