Enzymatic and non-enzymatic antioxidants changes in precancerous and cancerous lesions of oral cavity
Journal Title: Medpulse International Journal of Biochemistry - Year 2017, Vol 5, Issue 1
Abstract
Background: Involvement of ROS in the development of tobacco related oral carcinogens is speculated. Little is known about the status of ROS scavengers or antioxidant enzymes in association with precancerous and cancerous conditions of oral cavity. In view of this, we investigated the parameters of antioxidants (enzymatic and non-enzymatic) in lymphocytes which are part of the body’s immune system and more sensitive to oxidative stress. Material and Methods: The study involved the analysis of various parameters using established standard methods. Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxide (GSH-Px), Glucose 6 phosphate dehydrogenase (G6PD) activities and Reduced Glutathione(GSH) were determined in the blood lymphocytes of patients with precancerous lesions (OSMF and Leukoplakia) and biopsy proven well differentiated Squamous cell carcinoma, enrolled in Oral Radiology department of Nair Dental College and Hospital and from healthy age and sex matched control. Plasma levels of non-enzymatic antioxidants β- carotene, Retinol, α-tocopherol and vit C and plasma levels of TBARS expressed as MDA was determined to find the extent of lipid peroxidation. Results: Enzyme activity was represented as mean ± S.D. and the data was analysed statistically. Student –t was applied to study the comparison of mean enzyme activity of healthy control group with patient groups and correlation coefficient (r) were computed using SPSS software. A highly significant decrease in the levels of SOD, CAT and GSH-Px G6PD and GSH (P<0.001) was noted in cancerous group. In precancerous cases all the enzymes showed moderate to significant fall in the activities. A moderate to significant fall in the plasma levels of β- carotene, retinol, vit c and α-tocopherol was noticed in both the groups as compared to the controls. MDA level expressed significant rise (P<0.001) in precancerous and cancerous group as compared to control. Conclusion: The antioxidant enzyme activity was reduced and lipid peroxidation was increased in oral precancerous and cancerous group.
Authors and Affiliations
Sarita Basu, V Naga Guhan
Keywords
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