Estradiol Regulates Insulin Dependent Stimulation of LDL-Receptor Expression in HepG2 Cells
Journal Title: Indian Journal of Clinical Biochemistry - Year 2006, Vol 21, Issue 1
Abstract
The low density lipoprotein, one of the major vehicle for extraneous cholesterol, internalizes into the cells through the process of LDL-receptor mediated endocytosis. The expression of LDL- receptor on the cell surface is a function of various hormone regulated transcription of the receptor gene. The present study elucidates the differential expression pattern of LDL-receptor protein in human hepatoma HepG2 cells by the influence of two hormones, insulin and estrogen (β-estradiol), as compared to the basal level expression of the receptor protein . The combined effect of insulin and β-estradiol reveals that β-estradiol is the ultimate regulator between these two hormones and supershades the message of insulin on LDL-receptor expression. The receptor protein level immunobloted by anti LDL-receptor antibody after treating cells with insulin, β- estradiol and mixture of both also reflects the same phenomena. This comparative study makes it consistent that cell saturation with sterol (β-estradiol) is the prime regulator of LDLreceptor expression between the two hormones , insulin and estrogen.
Authors and Affiliations
Gopalakrishnan R. and N. C. Chandra#
Keywords
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