EVALUATION ACTIVITIES OF CHITOSAN ASCORBATE AGAINST RODS OF HELICOBACTER PYLORI ISOLATED FROM GINGIVAL POCKETS AND ATHEROSCLEROTIC PLAQUES
Journal Title: Progress on Chemistry and Application of Chitin and its Derivatives - Year 2016, Vol 0, Issue
Abstract
The aim of the study was determine activity of chitosan ascorbate against 17 strains of Helicobacter pylori. The strains were isolated from gingival pockets (7 strains), atherosclerotic plaques from carotid and femoral (10 strains). Chitosan was obtained from krill chitin and deacetylation degree was equal 60%. The ratio of ascorbic to chitosan was equal 1. The susceptibility of rods was determined by means of plate dilution technique in Brucella agar with 5% sheep’s blood. The inoculum contained 105 CFU/spot. Incubation was performed in anaerobic jars with Campy Pak (BBL) for 48 hrs. The MIC was interpreted as the lowest concentration of chitosan ascorbate inhibiting the growth of bacteria. The results indicated, that chitosan ascorbate at the lowest concentrations was active against 35% of the strains. The Helicobacter pylori rods isolated from gingival pockets were the highest susceptible than isolated from atherosclerotic plaques.
Authors and Affiliations
Anna Kędzia, Barbara Kochańska, Alina Gębska, Maria Wierzbowska, Andrzej Kufel
Keywords
Related Articles
- EP ID EP190578
- DOI 10.15259/PCACD.21.10
- Views 85
- Downloads 0
THE EFFECT OF CHITOSAN ON THE SYNTHESIS OF L-S-NITROSOCYSTEINE THAT PARTICIPATES IN THE REGULATION OF THE M2 PYRUVATE KINASE ISOENZYME ACTIVITY ASSOCIATED WITH EHRLICH ASCITES CELLS PROLIFERATION IN VITRO
L-S-nitrosocysteine formation in EAT tumor cells and normal CRL-1636 cells incubated with microcrystalline chitosan was confirmed by RP-HPLC. The metabolite was identified based on UV-VIS spectra. The formation of L-S-ni...
ASSESSMENT OF PHARMACEUTICAL PROPERTIES OF THERMOSENSITIVE GYNECOLOGICAL POWDERS CONTAINING LACTIC ACID COMPLEXED WITH CHITOSAN
pH of gels ranged from 3.92 to 4.44 for gels 1:1 and from 2.36 to 2.84 for 8:1 ratio. The addition of 20%, 23% or 25% poloxamer 407 increases the pH ranged from 4.00 to 4.91 for gels with 1:1 ratio and from 2.56 to 3.42...
THE INFLUENCE OF THE TYPE SOLVENT ON THE STRUCTURE OF CHITOSAN BLENDS WITH HYALURONIC ACID
The influence of the type solvent on the structure of chitosan, hyaluronic acid and their blend films was investigated. Aqueous acetic acid, hydrochloric acid, sodium chloride and aqueous acetic acid/NaCl were used as so...
STRUCTURAL RESEARCH OF THERMOSENSITIVE CHITOSAN-COLLAGEN GELS CONTAINING ALP
Introducing collagen, which is basic ingredient of bone tissue, into the structure of chitosan gels which are formed at the physiological body temperature, is aimed at creating the so-called biomimetic structures, i.e. c...
STUDIES ON THE USABLE PROPERTIES OF INNOVATIVE WOUND DRESSINGS
The new implemented wound dressings should meet several requirements connected with the clinical safety and performance. The series of PN-EN 13726 Standards describes the tests methods for the verification the most impor...