Evaluation of the Effects of a High Dose of Erythropoietin-beta on Early Endotoxemia Using a Rat Model
Journal Title: Advances in Clinical and Experimental Medicine - Year 2012, Vol 21, Issue 3
Abstract
[b]Abstract[/b] [b]Background.[/b] Endotoxins can cause serious organ damage and death by triggering the secretion of pro-inflammatory cytokines such as TNF-α, IL-6 and IL-1β in bacterial infections. [b]Objectives. [/b]The goal of this study was to evaluate the effects of a high dose (3000 U/kg) of erythropoietinbeta (EPO) on inflammatory cytokine levels, renal function and histological changes during the early period of Lipopolysaccharide (LPS)-induced endotoxemia using a rat model. [b]Material and Methods.[/b] Male Sprague Dawley (350–400 g) rats were randomized into 3 groups: Control group (n = 7); LPS group (received 20 mcg/kg LPS through intraperitoneal (i.p.) injection (n = 7); LPS+EPO group (received 3000 U/kg, ip 30 minutes before LPS administration (n = 7). Four hours after the administration of LPS, kidney tissue and serum samples were collected. Kidney function parameters, TNF-α, IL-6, IL-1β, C reactive protein (CRP) and complete blood counts (CBC) were measured. The severity of renal tubular injury and caspase- 9 immunoreactive cells was expressed as a percentage. [b]Results.[/b] Serum levels of urea, creatinine, TNF-α, IL-6 and IL-1β were significantly increased in the LPS group (p < 0.0001 – p = 0.04) and were lower in LPS+EPO group (p < 0.0001, p = 0.01, p = 0.02, p = 01 and p < 0.0001, respectively). Pretreatment with EPO significantly increased platelet counts (p = 0.00) and decreased white blood cell counts (p = 0.02). The renal tubular injury percentage was significantly higher in the LPS group than in the control and LPS+EPO groups (p = 0.002, p = 0.003, and p = 0.005, respectively) and caspase-9 expression was lower in the LPS+EPO and control groups than in the LPS group. [b]Conclusions.[/b] EPO might have renoprotective effects against the inflammatory process and cell apoptosis during endotoxemia.
Authors and Affiliations
Zehra Eren, Jale Çoban, Işın Ekinci, Cigdem Kaspar, Gülçin Kantarcı
Keywords
Related Articles
- EP ID EP98006
- DOI -
- Views 147
- Downloads 0
Evaluation of the Effects of a High Dose of Erythropoietin-beta on Early Endotoxemia Using a Rat Model
[b]Abstract[/b] [b]Background.[/b] Endotoxins can cause serious organ damage and death by triggering the secretion of pro-inflammatory cytokines such as TNF-α, IL-6 and IL-1β in bacterial infections. [b]Objectives. [/...
The Potential Role of Photodynamic Therapy in the Treatment of Malignant Melanoma – an in vitro Study
[b]Background.[/b] Melanoma is the most severe of skin neoplasms as it may grow rapidly and metastasize. The application of photodynamic therapy (PDT) opens up new prospects in the treatment of this tumor. Numerous studi...
Endothelial Progenitor Cells in Diabetic Foot Syndrome
In the late 20th century endothelial progenitor cells (EPCs) were discovered and identified as cells capable of differentiating into endothelial cells. Antigens characteristic of endothelial cells and hematopoietic cells...
The Biological Role of Carnosine and Its Possible Applications in Medicine
The article reviews current literature on the biological role of carnosine, its properties and use as a supplement in periods of intense physical activity. Studies carried out on laboratory animals and humans have shown...
Analiza stężeń interleukiny 15 w płynie owodniowym w II i III trymestrze ciąży
[b]Wprowadzenie.[/b] Interleukiny odgrywają istotną rolę w regulacji odpowiedzi immunologicznej organizmu. Interleukina 15 (IL-15) jest glikoproteiną wydzielaną głównie przez makrofagi i monocyty. IL-15 odgrywa kluczową...