Expression of acrA gene in ciprofloxacin and tetracycline resistant Esherichia coli mutants with possible mutation in acrR
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2013, Vol 6, Issue 9
Abstract
In E. coli the major transporter responsible for the high intrinsic level of antibiotic resistance is AcrAB-TolC. The ingredients of pump are encoded by acrA, acrB and tolC. The expression of first two genes is inhibited by a repressor called AcrR. However, MarA activates the expression of three genes. Occurrence of a mutation in marR and acrR can lead to overexpression of the pump. The aims of this research were to use ciprofloxacin and tetracycline resistant mutants having a mutation in marR gene for studying first, the possible presence of mutations in acrR gene and then the expression of acrA. For these purposes the DNA binding region of acrR gene in these mutants were amplified and sequenced, and the relative expression of acrA in these mutants was quantified by real time PCR. Results showed that one of the mutant and its clone had a mutation in acrR, but none of the mutants overexpresses acrA in comparison with wild type strain. The effect of marR and acrR mutations on acrAB overexpression is dependent on levels of resistance to tetracycline and ciprofloxacin.
Authors and Affiliations
Razieh Pourahmad Jaktaji, Nasim Jazayeri
Keywords
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