Fatty Acid Methyl Ester Analysis of Olive Oil Degraded by Pseudomonas fluorescens
Journal Title: Journal of Advances in Microbiology - Year 2017, Vol 2, Issue 2
Abstract
Fatty acids are the major components of lipids; and the physical, chemical and physiological properties of a lipid class depend primarily on its fatty acid composition. Gas chromatography method can be used for the identification of microbiological degraded fatty acids in vegetable oils as methyl ester. In this work, olive oil degraded by lipase of Pseudomonas fluorescens grown in two different Mineral Salt Medium, was studied over 25 days. Olive oil was analyzed for fatty acids commonly present in olive oils which are Myristic, Palmitic, Stearic, Oleic, Linoleic, Linolenic, Behenic and Lignoceric, which have specific carbon number and their values in approximate percentage are C14:0 (0.4), C16:0 (14.0), C18:0 (5.5), C18:1 (76.4), C18:2 (3.4), C18:3 (0.1), C22:0 (0.1) and C24:0 (0.1) respectively. Oleic acid percentage is high in olive oil which contained considerable amount of 76.4%. The reduction of fatty acid by Pseudomonas fluorescens after 20 days was 8.2% in the media used. Lipase from Pseudomonas fluorescens had potential for degradation of fatty waste. It could therefore be employed in environmental cleanup of oil spill site.
Authors and Affiliations
B. M. Popoola, A. A. Onilude, T. Olateru Comfort
Keywords
Related Articles
- EP ID EP338171
- DOI 10.9734/JAMB/2017/31239
- Views 81
- Downloads 0
Prevalence of Salmonella typhi Infection in Karu Local Government Area of Nasarawa State, Nigeria
Aim: The aim of this study is to determine the prevalence of Salmonella typhi infection in Karu Local Government Area of Nasarawa State, Nigeria. Place of Study: Karu Local Government Area, Nasarawa State; Department of...
Utilization Potential of Kitchen Waste Sludge as Organic Fertilizer
Aim: To investigate the possible conversion of kitchen waste sludge into organic fertilizer using a bio-treatment agent. Place and Duration of Study: The study was carried out at Afe Babalola University in the green hous...
In-vitro Anti-Bacterial Activity of Extracts of Euphorbia abyssinica (Desert Candle) Stem-Bark and Latex
Aim: This study was aimed at evaluating Euphorbia abyssinica (Desert Candle), a medicinal plant extensively used in folklore medicine among the Kendem people of South-west Cameroon for antibacterial activity and extracts...
The Prevalence of Skin-tattooing and HIV among Students of Three Tertiary Institutions in Ondo State, Southwest, Nigeria
With the huge potential health hazards and dangers associated with skin and sclera tattooing, there is hardly any responsible government anywhere that will not take interest in the rate and manner its populace in general...
Isolation and Antibiogram of Bacteria Isolated from Processed and Unprocessed Cow-Skin (Ponmo) in Ogbese Market
The use of ponmo as major meat source to average Nigerians if processed under unhygienic condition might result in disease outbreak. Inadequate and under-utilization of food nutrients needed by human for normal body func...