Field evaluation of genetic stability in plants obtained by somatic embryogenesis of cv. ‘FHIA 21’ hybrid (Musa AAAB)
Journal Title: Biotecnología Vegetal - Year 2007, Vol 7, Issue 3
Abstract
The present work was carried to evaluate the genetic stability in plants obtained by somatic embryogenesis of cv. hybrid ‘FHIA 21’ (Musa AAAB) in field. A number of 6 252 plants were planted and 2 000 plants obtained by organogenesis and same quantity coming from asexual seeds were used as control for this study. The plantation density was 3 333 plants per hectare, at a distance of 3x2x1.20m each one. Four repetitions were carried out in a Latin Square experimental design. Evaluations were made during the first plantation cycle through out morphological and agronomic characters of more contribution to detection of genetic variability. Results showed high genetic stability in plants obtained by somatic embryogenesis of cv. hybrid ‘FHIA 21’ (Musa AAAB), given by the low percentage of somaclonal variation (0.015%). Besides, the evaluation of the agronomic characters (bunch weigh, number of hands/bunch and number of fruits/hands) did not present statistical differences among the plants coming from the in vitro culture (somatic embryogenesis and organogenesis), but some differences were observed when compared to plants from asexual seeds. Results of this work validate somatic embryogenesis for the massive propagation of cv. hybrid of banana ‘FHIA-21.’
Authors and Affiliations
Leyanis García-Águila, Miladys León, Rafael G. Kosky, Pedro Orellana, Ricardo González
Keywords
Related Articles
- EP ID EP684098
- DOI -
- Views 146
- Downloads 0
Detection and quantification of quitinasa in banana (Musa spp.) leaves inoculated with Mycosphaerella fijiensis
The knowledge of the role of chitinase enzyme in Musa spp- Mycosphaerella fijiensis interaction is still very limited. Therefore, their study is of great interest, mainly in breeding programs. The aim of this study was t...
Field evaluation of regenerated plants by somatic embryogenesis from shoots apexes from axilary buds in cv.‘Navolean’ (Musa spp., AAB)
The use of shoots apexes from axilary buds for callus induction with embryogenic structures in plantain ‘Navolean’ (Group AAB) permitted to develop a plant regeneration method through out somatic embryogenesis. In order...
Antioxidant enzymes preserving coffee quality in refrigerate environment
Post-harvest phases may cause biochemical and physiological changes with direct effects on coffee (Coffea sp.) grains quality during its storage. The aim of this study was to determine the relevance of antioxidant isoe...
Genetic transformation by Agrobacterium tumefaciens of embryogenic cell suspensions of banana cultivar Grandenaine (AAA)
The black Sigatoka (Mycosphaerella fijiensis Morelet) has become in the last years, the most destructive disease that affects the production of banana and plantains world-wide. The present work was made with the objecti...
Detection of biochemical compounds related to defensive response in Musa spp. plants inoculated artificially with Mycosphaerella fijiensis
Knowledge about biochemical events involved in both compatible and incompatible interaction in Musa-M. fijiensis pathosystem it is still very limited. Then, the use of tools to study fast and simply plant defence mecha...