FINGERPRINTING GENOMES OF UKRAINIAN-BREED CULTIVARS OF SWEET CHERRY BY USING IRAP AND REMAP MARKERS
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2014, Vol 6, Issue 2
Abstract
The using of uniform plant material with confirmed cultivar identity is the most critical factor for creating gardens of intensive type. The efficiency of identification method and analysis of genetic diversity between different fruit cultivars with traditional phenotypical traits are insufficient taking into account its high dependence from environmental factors. Genomes fingerprinting with molecular-genetic markers is a most accurate and reliable method of eukaryotic genomes studying. Retrotransposon markers have been demonstrated to be powerful tools for genetic fingerprinting of eukaryotic genomes because they are the most abundant mobile elements in the plant genome and seem to play an important role in genome reorganizations induced by natural and artificial selection. The problem of searching of most informative marker systems for genetic fingerprinting genomes of sweet cherry cultivars is always actual due to rather low level of genetic polymorphism in this culture. This is a reflection of limited genetic variability of sweet cherry germplasm due to system of gametophytic self-incompatibility. In our investigation 13 Ukrainian sweet cherry cultivars and two foreign cultivars as a control were analyzed. Total genomic DNA was extracted by CTABmethod with some modification. PCR was conducted in IRAP- and REMAP- modifications. Amlification products were separated in PAAG. A discriminative possibility of marker systems used was assessed by different indices of polymorphism for comparing their informative content. This study allowed to evaluate discriminative possibilities and technical peculiarities of some systems for molecular-genetic fingerprinting of Ukrainian sweet cherry cultivars using IRAP- and REMAP-PCR markers and to select among them the most perspective ones for routine analysis. Applying of five primers (IRAP-PCR) and two combinations of primers (REMAP-PCR) allowed to obtain 63 markers, among which 23.8 % were polymorphic. In was demonstrated that for genetic fingerprinting of sweet cherry cultivars the application of primers combination TRIM K002 +8565 and single primers TRIM K002, iPBS 2272 and iPBS 2077 are perspective.
Authors and Affiliations
Я. І. ІВАНОВИЧ, Н. В. ТРЯПІЦИНА
Keywords
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