Fungal Detoxification of Certain Fusarium Moniliforme Mycotoxins Using Yarrowia Lipolytica with Special Emphasis on its Possible Mechanism of Action
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 1
Abstract
Detoxification of mycotoxins must be taken to ensure food and feed safety. The aim of this study was to determine the influence of Yarrowia lipolytica on mycotoxin elimination and inhibition of Fusarium moniliforme growth. In this study, Y. lipolytica could remove 84.92% of Fuminosin B1 (FB1) and 80.64% of zearalenone (ZEN) from potato dextrose broth medium. Yeast cells and culture broth decreased FB1 by 34.16% and 82.59%, along with decreasing ZEN by 51.83% and 75.34%, respectively. Optimization results showed highest degradation percentage after incubation of 0.6g yeast cells for 96h at 37°C, pH 6 in the presence of glucose and peptone as C and N sources. Likewise, incubation with Mg2+ and Se2+ ions significantly increased toxin degradation. Proteinase K, EDTA and heat treatment decreased degradation activity, proving that the degradation is enzymatic. TEM observation confirmed the inhibitory effect of Yarrowia broth on F. moniliforme, indicating that inhibition of the toxin-producing strain is another mechanism of action. Conclusively, the applied yeast has detoxification capability of mycotoxins and especially FB1 due to more than one mechanism. This study spotted the light on Yarrowia lipolytica strain that can be used to safely remove ZEN or FB1 toxicity from food and animal feed.
Authors and Affiliations
Elaasser, Mahmoud Mohamed;, Magdi, Hani Mohamed, Mourad, Mohamed Hosny;, Ahmed, Hanaa Yousef
Keywords
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- EP ID EP388354
- DOI 10.9790/3008-1201046373
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