Genetic diversity assessment using RAPD primers in insecticide resistant populations of diamondback moth Plutella xylostella (Linn.)
Journal Title: Journal of Applied and Natural Science - Year 2015, Vol 7, Issue 1
Abstract
Genetic diversity in acephate, spinosad and Cry2Ab resistant Plutella xylostella collected from three states of India was assessed by RAPD markers. The DNA extracted from larvae was subjected to polymerase chain reaction using 10 RAPD primers. The highest number alleles (7) were produced by primer ABA-13, followed by six alleles each by primers ABA-2, 7, 8, 11, 14; five alleles each were produced by ABA-4, 9, 10, 12. UPGMA analysis clustered the acephate, spinosad and Cry2Ab treated P.xylostella populations into two groups with overall similarity level of 33%, 27% and 34% respectively. Cluster A consisted 11 samples while Cluster B consisted only F1 of acephate and spinosad treated Karnataka population. In Cry2Ab treated population Cluster B comprised 11 samples and Cluster A had out grouped singly i.e. F0 generation from Karnataka. The genetic variability between the acephate, spinosad and Cry2Ab treated populations ranged from 33 to 69%, 27 to 56% and 34 to 69% respectively. Acephate and spinosad treated F1 population and Cry2Ab treated F0 population from Karnataka were out grouped from rest of the populations.
Authors and Affiliations
V. Sunitha, T. V. K. Singh, V. Ramesh Babu, J. Satyanarayana
Keywords
Related Articles
- EP ID EP282914
- DOI 10.31018/jans.v7i1.593
- Views 104
- Downloads 0
Performance of blackgram (Phaseolus mungo L.) cultivars as influenced by row spacings and molybdenum
A field trial was conducted to assess the performance of blackgram cultivars to row spacings and molybdenum doses at the Instructional-cum-Research Farm, Indira Gandhi Krishi Vishwavidyalya, Raipur (Chhattisgarh), under...
Economic loss assessment on juvenile fish catch due to forced non-selectivity in a selective fishing gear, gillnet along Mumbai coast, India
A study on quantity and value of juvenile fish landings was carried out in the gillnet fishery of three selected landing centers along Mumbai coast viz., Versova, Cuff Parade and Mahim in India using Out board motors (OB...
Isolation and characterization of flavone di-glucoside and acetoxyxanthone from the flowers of Bombex ceiba
In present study two chemical constituents viz. Flavon 5,4’ dimethoxy 8 methyl 7-O- β -D glucopyranoside-5’- β -D-glucopyranoside and xanthone 3-acetoxy-1-hydroxy-6-methoxy8-O– β –D-glucopyranosyl-(1 → 3)- α –L- rhamnopy...
Genetic diversity analysis for various morphological and quality traits in bread wheat (Triticum aestivum L.)
The present investigation was carried out during 2011-12 in a randomized block design (RBD) with 35 diverse wheat genotypes to assess the genetic diversity for various morphological and quality traits. The analysis of va...
Isolation and screening lactic acid bacteria for riboflavin production and their use for bioenrichment of curd
As many as 47 lactic acid bacteria were isolated from various vegetables and fruits and raita collected from local households and characterized. All of them were Gram positive and catalase negative. The isolates were scr...