GENETIC POLYMORPHISM ANALYSIS OF STRAWBERRY (FRAGARIA ANANASSA DUCH.) USING SSR-MARKERS
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2017, Vol 9, Issue 1
Abstract
Elaboration of effective and reliable assessment methods for genetic polymorphism of varieties, hybrids and initial forms with using molecular markers is an important tool in selection process, planting material certification and copyright protection. Estimation of genetic polymorphism for strawberry varieties of Ukrainian and worldwide selection with SSR-analysis was carried out in the research. Awareness of 12 SSR-loci was analyzed from scientific sources. 9 loci which made it possible to obtain explicit and reproducible alleles were selected for further analysis of strawberry varieties. 3 microsatellite loci weren’t used due to the presence of diffuse bands and problems with interpretation of alleles with large number. Totally 69 alleles were detected in strawberry varieties using 9 selected loci. Molecular size of alleles was 278-76 bp. The largest amount of alleles was obtained for amplification EFMvi136 locus (11 alleles). The smallest amount for EMFv029 locus (4 alleles). The highest level of heterozygosity was found for EMFvi108 locus (43.2 %). The lowest level for UDF002 locus (10.1 %). The range of genetic distances between varieties ranging from 0.059 (between Holosiivska rannia – Sweet Ann) to 0.65 (between Sweet Ann – Senga Sengana and Holosiivska rannia - Elsanta). Indices of genetic identity ranging from 0.521 to 0.942 between the same varieties. A cluster analysis was carried out and the dendrogram of genetic affinity was constructed by UPGMA (Unweighted Pair Group Method with Arithmetic Mean). According to the SSR analysis, the varieties are clearly divided into two separate clusters. The first cluster included varieties of Ukrainian and American origin. The closest pairs by genetic distances were Holosiivska rannia – Sweet Ann (d=0.059), Berehynia – Fakel (d=0.139), Albion – Alina (d=0.208). The second cluster included varieties of Italian, Holland and German origin: Clery – Alba (d=0.245) and Elsanta – Senga Sengana (d=0.263). The results of 9-SSR analysis allowed a revealing polymorphism by all molecular-genetic markers. The level of polymorphism varied depending on SSR-marker. Genetic identification system of strawberry varieties was proposed based on input data
Authors and Affiliations
О. В. ДУБІН, М. Д. МЕЛЬНИЧУК, А. Ф. ЛІХАНОВ
Keywords
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