Genetic polymorphisms of GSTM1 and GSTT1 and susceptibility to primary DNA damage in steel industry workers
Journal Title: International Journal of Life Science - Year 2018, Vol 6, Issue 4
Abstract
The present study aims to investigate the possible association of detoxifying gene polymorphisms with DNA damage in steel industry workers. 150 steel industry workers and 146 control subjects with no history of occupational exposure to steel dust or any other chemicals were recruited for the study. DNA damage was evaluated using simple and reliable alkaline comet assay in peripheral blood lymphocytes of male steel industry workers. Analysis of GSTM1 and GSTT1 gene polymorphisms was done by multiplex PCR method. The results showed an increase in the frequency of DNA damage in peripheral blood lymphocytes of the steel industry workers compared to the control subjects. The statistical analysis of the results showed that significant DNA damage was observed in both genotypes of GSTM1 and GSTT1 but the frequency was found to be lower in steel industry workers with wild GSTM1 and GSTT1 genes than in those with null genotypes. The findings in this study presented evidence for the DNA damage highlighting an increased genotoxic risk as a result of exposure to dust in steel industry workers. The study also presented evidence for an association of both null genotypes of GSTM1 and GSTT1polymorphisms with DNA damage indicating the influence of GSTM1 and GSTT1 polymorphisms on this biomarker.
Authors and Affiliations
U. Indira Priyadarshini, Vanitha B. , Ch. Prashanth, Sridevi T. , Priyanka P. , P. Pranay Krishna, Latheef SAA, P. P Reddy
Keywords
Related Articles
- EP ID EP545820
- DOI -
- Views 83
- Downloads 0
Molecular detection and characterization of Canine distemper virus from domestic dogs (Canis familiaris) in Quezon City, Philippines
Canine distemper (CD) is a highly infectious viral disease affecting all terrestrial carnivores worldwide. In spite of the availability of commercial modified live vaccines, several studies have reported the occurrence...
Anti-inflammatory potential of flavonoids from Hemigraphis colorata
Agents that inhibit inflammatory mediators are highly recommended in the development of anti-inflammatory drugs. Flavonoids are large class of secondary metabolites around 10,000 structures, possess anti-inflammatory an...
An evaluation of comparative biosorption study of Cadmium (II) and Chromium (VI) using Orange rind (Citrus sinensis), (L.) Osbeck, under optimized conditions
The present study investigated the comparative biosorption study of cadmium (II) and chromium (VI) using inexpensive biosorbentrind of orange (Citrus sinensis), (L.) Osbeck, under optimized conditions. A biosorption stud...
A study on mutagen induced development of antibiotic resistance in some pathogenic bacteria
The evolution of antibiotic resistance by pathogenic bacteria poses a major task for human health. Whereas it is unambiguous that natural selection promotes resistance-conferring mutations, our understanding of the respo...
Congregations of two subspecies of Egyptian Vulture Neophron percnopterus in Unnao district of Uttar Pradesh, India
The Globally Endangered Egyptian Vulture Neophron percnopterus congregates in the areas which are away from human disturbances, where food availability is high and roosting substrates are present. There are three subspec...