Genotypic pathogenic microbes associated with deterioration of sweet orange (Citrus sinensis L) fruit in Nigeria
Journal Title: Scientia Agriculturae - Year 2016, Vol 13, Issue 1
Abstract
This study was carried out in Osogbo township of Osun State, Nigeria to isolate and determine prevalence and pathogenicity of microorganisms associated with deterioration of sweet orange fruits. Twenty samples of 20 infected and 20 non-infected sweet oranges (Citrus sinensis L) were collected from four open markets (Akindeko, Igbonna, Oja-Oba and Sabo markets) each. The samples were transported immediately to Fountain University Microbiology Laboratory for pathogenic analysis. The oranges were rinsed with distilled water and serially diluted in 10 folds. The highest three dilutions were considered for microbial count analysis. Each of the orange was cut and the liquid content inoculated on nutrient agar and potato dextrose agar, incubated at 370C and 250C respectively. They were observed for seven days, and the different colonies isolated using the slide culture technique. Biochemical analyses of the culture showed that Apergillus spp, Staphylococcus spp, Escherichia spp, Rhizopus spp and Shigella spp had the highest load. Pathogens prevalence revealed that Staphylococcus spp had highest (12.63%) at Sabo, 4.94% at Igbonna and 10.43% at Akindeko. Aspergillus spp with 6.60% and 17.58% loads were identified at Sabo and Oja-Oba respectively. Rhizopus spp had 21.97% at Oja-Oba, E. coli, 17.58% at Igbonna and Shigella spp, 8.24% at Akindeko. Rhizopus spp and Aspergillus spp were the most active microbes with respective 100% and 90% infections, while the least active microbes were Staphylococcus spp and Shigella spp. Harvesting fruits at the suitable periods and stored the harvested orange fruits under controlled conditions could aid in retarding the microbial growth of post-harvest spoilage of pathogenic microorganisms.
Authors and Affiliations
Omolaran B Bello, Odunayo J Olawuyi, Opeyemi S Adebisi, Alafe H Azeez, DT Oladimeji
Keywords
Related Articles
- EP ID EP159212
- DOI 10.15192/PSCP.SA.2016.13.1.1922
- Views 118
- Downloads 0
Feeding efficacy of coccinella septempunctata and Propylea quatuordecimpunctata against Macrosiphum rosae.
The lady bird beetle (Coccinellidae: Coleoptera) is being exploited as a potent animal for the effective control of the aphids besides other animal pests that cause colossal economic losses throughout Pakistan.The...
Performance of Some Zinc Enriched Rice Genotypes in Different Agro-Ecological Conditions of Bangladesh
In Bangladesh, many people especially the children and pregnant woman are used to suffer from malnutrition. The children become stunted growth which might be due to deficiency of micronutrients specially the zinc....
Effects of tuberculosis on sugarcane farmers productivity in the lubombo region of swaziland
The study examines the effects of tuberculosis on sugarcane’s farmers’ productivity in the Lubombo region of Swaziland. One hundred and forty sugarcane farmers were selected for the study, through stratified random...
Effect of N fertilization and plant density on yield and yield components of grain sorghum under climatic conditions of Sistan, Iran
In order to study the effect of different levels of N fertilization and plant density on yield and yield components of grain sorghum cv. Sistan, an experiment was conducted in agricultural research farm of Zehak, I...
Combined effect of solar drying and gamma radiation on the microbiological quality of mushrooms (Pleurotus ostreatus (Ex.Fr.)
The microbiological quality of dried Pleurotus ostreatus stored at room temperature was evaluated after solar drying and gamma irradiation at low doses of 0 (control), 0.5, 1, 1.5 and 2 kGy at a dose rate of 1.7 k...