IMMUNOPROTEOMIC ANALYSIS OF SECRETORY PROTEINS OF ASPERGILLUS FUMIGATUS WITH SPECIFIC IGE IMMUNOREACTIVITY
Journal Title: Indian Journal of Clinical Biochemistry - Year 2006, Vol 21, Issue 2
Abstract
Allergenic/antigenic proteins are known to induce Type I and Type III hypersensitivity reactions leading to allergic bronchopulmonary aspergillosis (ABPA) in immunocompetent host. The common structural features or intrinsic properties of the allergens/antigens leading to allergenicity in a host are not well understood. In the current report, comparative analysis of proteins on two dimensional gel electrophoresis (2D-E) and specific IgE immunoblots of A. fumigatus secretory proteins (1st, 2nd and 3rd week culture filtrate proteins) was carried out. We observed a total of 159 proteins in 1st, 2nd and 3rd week culture filtrates of A. fumigatus. Specific IgE immunoreactivity was observed in 75 proteins with different intensity. Third week culture filtrate showed maximum number of proteins, 142, and specific IgE immunoreactive proteins, 65. MALDI-TOF analysis resulted in putative identification of two allergens as hypothetical protein YBL057c from Saccharomyces cereviseae and unnamed protein product from Debaryomyces hansenii (similar to IPF14568 of Candida albicans). Identification of a repertoire of specific IgE immunoreactive proteins will facilitate the studies on structurefunction relationship of these proteins relevant for diagnosis and pathogenesis.
Authors and Affiliations
Poonam Gautam, Taruna Madan, W. Gade, P. Sarma
Keywords
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