IN VITRO ESTABLISHING OF POPLAR AND WILLOW CLONES PERSPECTIVE FOR RENEWABLE ENERGETICS
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2017, Vol 9, Issue 1
Abstract
In vitro microclonal propagation of woody plants is an important biotechnological approach for their propagation. It allows to produce the clonal material of high quality and consequently to increase the productivity of plantations. For successful in vitro culture initiation of fast growing poplars and willow hybrids, the effectiveness of plants sterilization and survival were evaluated with the using various sterilization treatments and nutrient media. The buds from fast growing hybrids of willow clones: “Zhytomyrska-1”, “Lukash”, “Olimpiysky vohon”, “Pechalna” and poplars clones: “Lubenska” and “Kelibirdynska” were washed with different solutions – concentrated soap solution, sodium hypochlorite and 70% ethanol during different exposure time and then planted on nutrient MS and WPM media supplemented with different combinations of phytohormones. Highly efficient sterilization (78%) was achieved with the using three-step treatment of soap solution, sodium hypochlorite and 70% ethanol with exposure time of 2, 10 and 1 min respectively. When plants were cultivated on WPM media supplemented with 0.3 mg/l BAP and 0.1 mg/l IBA, high rate of rhizogenesis and induction of meristems were determined. Average rooting rate obtained in the research was 93%. All studied clones have been successfully established into in vitro culture, except poplar clone “Kelibrdynska”, what probably needs another protocol for cultivation. In vitro established plants will be later used for genetic engineering studies.
Authors and Affiliations
Л. В. ХУДОЛЄЄВА, Н. К. КУЦОКОНЬ, О. Г. НЕСТЕРЕНКО, Н. М. РАШИДОВ, О. М. ДУГАН
Keywords
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