In vitro establishment and multiplication of Podocarpus oleifolius D. Don
Journal Title: Biotecnología Vegetal - Year 2015, Vol 15, Issue 2
Abstract
Romerillo (Podocarpus oleifolius D. Don) is the only endemic conifer of Ecuador and is considered endangered. The objective of this research was to establish and to in vitro multiply of P. oleifolius via direct organogenesis from apical segments of young trees grown in field. For disinfection, four concentrations of sodium hypochlorite (NaOCl) (1.0, 1.25, 1.5 and 1.75%) for 10 min and then 70% ethanol for 30 s were studied. At four weeks of culture, the number of necrotic explants contaminated, viable (survival) and mortality was quantified. Furthermore, the effect of the MS salts concentration (50 and 100%) and Benzyl amino purine (BAP) (0.5 and 1.0 mg l -1 ) was determined. At four weeks of culture the apical bud length (cm) was measured and the number of sprouted buds was quantified. To in vitro multiply obtained romerillo sprouts, it was added different concentrations of BAP and Indol Butyric Acid (IBA) to the culture medium. The number of shoots formed per explant was quantified and the length of the apical bud (cm) was measured in three subcultures. With 1.25% NaOCl the highest percentage of viable explants with low percentages of microbial contamination and necrotic explants was obtained. The higher percentage of sprouted buds was obtained in explants grown in culture media without BAP and the highest values of the apical bud length in MS medium. In vitro multiplication of P. oleifolius it was achieved. However, growth regulators BAP and AIB at concentrations tested did not increase the number of shoots per explant. This work serves as a guideline for the implementation of a system for large-scale propagation of this plant species.
Authors and Affiliations
Mireya Galárraga , Jaime Hidrobo , Norman Soria , Jaime Gía
Keywords
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