In vitro establishment of apex and nodule segments of Swietenia macrophylla King
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 3
Abstract
The application of tissue culture techniques in woody plants offers a valuable alternative for the propagation of elite trees. With the objective of obtaining the in vitro establishment of mahogany (Swietenia macrophylla King), the starting material were young buds taken from plants in field conditions. For the disinfection of the vegetal material two concentrations of hypochlorite of sodium (NaClO) combined with three times of exposition were studied. The percentage of the variables contaminated explants, necrotic explants and survival, were evaluated. Later, the method of disinfection of the explants was determined. The nodules segments and apical buds were cultivated under environmental conditions of controlled light and temperature, in a culture medium of MS with reduction of nitrates to the half and supplemented with five concentrations of cytokinin (6-BAP). In all the tests forty repetitions by treatment were made and one explant by essay tube with 10 milliliters of culture medium. The evaluations were carried out at the 28 days and the percentage of the variables explants sprouted and average length of the bud in (cm) were evaluated. For the disinfection of the explants the suitable concentration of NaClO and the time of exposition of the explants to the same one were determined. The establishment of vigorous shoots of mahogany was obtained with a concentration of 0.2 mg.l -1 6-BAP with the use of nodular segments as explants, initial step of a direct propagation.
Authors and Affiliations
Raúl Collado, Raúl Barbón, Daniel Agramonte, Felipe Jiménez, Martha Pérez, Odalys Gutiérrez, Daymí Ramírez.
Keywords
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