Incidence of Methicillin Resistant Staphylococcus aureus and its antibiotic susceptibility pattern in a tertiary care hospital
Journal Title: Indian Journal of Microbiology Research - Year 2017, Vol 4, Issue 1
Abstract
A total of 250 Staphylococcus aureus strains were isolated from various clinical samples over a period of 9 months at M. P. Shah Medical College, Jamnagar to identify the incidence rate and antibiogram of Methicillin Resistance Staphylococcus aureus (MRSA). All strains of S. aureus were tested for susceptibility to methicillin / oxacillin by disk diffusion method and minimum inhibitory concentration (MIC) of methicillin was determined by agar dilution method as per standard procedures. Antibiogram study of MRSA strains was performed. Out of total 250 S. aureus strains, 145 (58%) strains were methicillin and oxacillin resistant, having methicillin MICs of ≥ 16 µg / ml, 100 (40%) strains were methicillin and oxacillin susceptible having methicillin MICs of ≤ 4 µg /ml. 5 (2%) strains were borderline susceptible having methicillin MICs of 8 µg / ml. Borderline susceptible strains were susceptible to co-amoxiclave and were not considered as methicillin resistant strains. All 145 (100%) MRSA strains were resistant to penicillin G, 139 (96%) were resistant to erythromycin, 136 (94%) were resistant to gentamicin, 80 (55%) were resistant to amikacin, and 35 (24%) were resistant to rifampicin. All MRSA strains were susceptible to vancomycin.
Authors and Affiliations
Sanjay Mehta
Keywords
Related Articles
- EP ID EP293638
- DOI 10.18231/2394-5478 .2017.0008
- Views 69
- Downloads 0
Mechanisms of bacterial degradation of arsenic
Arsenic is a toxic metalloid that exists in two major forms (arsenate and arsenite). The anthropogenic activities of man are its major source in the environment. Bacteria have developed resistance against arsenic by usin...
Correlation of VITEK -2 and conventional tests in identification of Non Fermenting Gram Negative Bacilli in a Tertiary Care Hospital
Introduction: The non-fermentative Gram negative bacilli (NFGNB) are a group of aerobic, non-spore forming, Gram negative bacilli that either do not utilize carbohydrates as a source of energy or degrade them through met...
Identification of emerging Chryseobacterium indologenes sepsis using automated identification system, Matrix-assisted Laser Desorption Ionization-time-of-flight Mass Spectrometry (MALDI-TOF) and Pulsed-field Gel Electrophoresis (PFGE) typing
Chryseobacterium indologenes is a non-motile, catalase-positive, oxidase-positive, indole-positive, non-glucose-fermenting Gram-negative bacillus that produces yellow coloured colonies on routine culture media. As common...
Detection of oxacillanase and metallobetalactamase in carbapenem resistant isolates of Acinetobacter spp., from various clinical samples by phenotypic and genotypic methods in a tertiary care hospital
Background: Non fermenting Gram Negative Bacilli (NFGNB) once considered as contaminants, now emerged as a major cause of life threatening nosocomial infections and as multidrug resistant pathogens. Acinetobacter species...
Utility of ADA (Adenosine Deaminase) enzyme assay in diagnosis of tuberculous meningitis
Introduction: Tuberculous meningitis (TBM) remains one of the leading causes of morbidity and mortality worldwide, including India. Treatment and prevention of TBM is depended on early and quality assured diagnosis. Earl...