Infectious Differences between the Dermatophytes-Induced Dermatophytosis in Wistar Rat
Journal Title: Journal of Applied Life Sciences International - Year 2016, Vol 5, Issue 3
Abstract
Aims: This study investigates the infectious differences between the two dermatophytes Trichophyton rubrum (T. rubrum) and Epidermophyton floccosum (E. floccosum), and analysed their growing effects and morphology on the skin of Wistar rats. Study Design: The histology and histochemistry studies were investigated. Methodology: Nine animals were used and divided into 3 equal groups. Group A was the control (not infected), group B was infected with T. rubrum and group C was infected with E. floccosum. 2 ml of washed fungal organisms were spilled on shaved, cleaned and disinfected skin of the Wistar rats with cotton swab saturated with 70% ethanol separately. The dermatophytes were allowed to incubate with noticeable changes when physically observed. Animals were sacrificed using cervical dislocation under chloroform anaesthesia, after 12 days of infection for both histological and histochemical studies. Results: The skin of the animals were observed with the falling of furs on the skin of the Wistar rats infected with different organisms compare with the control group. The histochemical studies of melanoaldehyde (MDA), superioxide dismutase (SOD) and total protein (T.P) were carried out with T. rubrum infected group observed to have highest level of SOD that is 778.02±4.00 as against 741.01±4.00 of E. floccosum, which suggested some protecting reactions in the skin against reactive oxygen species. Also, the total protein level of the infected groups increased slightly compare to the control group. The little increase in the level of MDA was observed in E. floccosum infected group. The histological appearance in the cellular architecture and arrangement in group B compared with group C with obvious differences observed. Thus, T. rubrum infected group digested keratin more than E. floccosum-infected group, and may be as a result of serious invasion of the epidermis and its rete ridges by the fungi. Conclusion: It can therefore be concluded that, T. rubrum is more infectious in terms on its commonest and keratin digestion when compared with E. floccosum.
Authors and Affiliations
S. G. Ojuolape, A. O. Muhammed, A. M. Afodun
Keywords
Related Articles
- EP ID EP351939
- DOI 10.9734/JALSI/2016/26250
- Views 72
- Downloads 0
Growth and Haematological Characteristics of Broiler Starter Birds Fed Processed Taro Cocoyam (Colocasia esculenta) Corm Meal
Aims: Broiler production fulfils important socio-economic functions, but inadequate nutrition, scarcity and high cost of feedstuffs in developing countries undermines broilers in expressing their full potential. Therefor...
Assessment of Changes in Beta-carotene Content and Sensory Attributes of Two Sweet Potato Varieties as Influenced by Organic and Inorganic Fertilizers and Storage Methods
Two field experiments were conducted at two seasons at the research fields of the University of Education, Winneba-Mampong campus from September, 2011 to January, 2012 and April to July, 2012 to assess changes in beta-...
Cuticular Modifications in Colocasia esculenta (L.) Schott. and Xanthosoma maffafa (L.) Schott. Accessions Exposed to Some Oilfield Chemicals in the Niger Delta, Nigeria
Aim: To evaluate the effects of sodium thiosulphate and graphite powder on the epidermal characters of Colocasia esculenta and Xanthosoma maffafa accessions. Study Design: The Randomized Complete Block Design was used f...
Oesophageal and Gastric Morphology of the African Rope Squirrel Funisciurus anerythrus (Thomas, 1890)
Aim: The aim of this study is to evaluate the gross, histological and histochemical features of the oesophagus and stomach of the African rope squirrel (Funisciurus anerythrus) were studied. Study Design: Experimental mo...
Effect of Environmental Factors on Biodecolourization of Textile Effluents Using Mutagenised Strains of Pseudomonas and Bacillus Species
Aim: To determine the effects of varying environmental factors on textile effluents biodecolourization ability of mutagenised strains of Bacillus and Pseudomonas species isolated from dye contaminated soil. Study Design:...